中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 卵泡刺激素剂量依赖性表达基因的筛选 浙江大学学报(农业与生命科学版) 2018 (2) 162-171
Position: Home > Articles > Screening of follicle-stimulating hormone dose-dependent expression genes Journal of Zhejiang University (Agriculture and Life Sciences) 2018 (2) 162-171

卵泡刺激素剂量依赖性表达基因的筛选

作  者：
刘甜;陈晓宇;朱志伟;于福先;黄菁;贾若欣;石放雄;潘建治
关键词：
卵泡刺激素;卵巢颗粒细胞;生物学效价;猪
摘  要：
为开发能高效检测卵泡刺激素(follicle-stimulating hormone,FSH)制品生物活性的新方法,选取猪卵泡颗粒细胞,利用转录组测序技术对比FSH处理组(40 m IU/m L)和空白对照组细胞中基因的表达情况,分析并获得大量受FSH调控的候选基因,运用荧光定量聚合酶链式反应验证FSH处理后显著上调的基因,并进一步利用FSH浓度梯度(0、20、40、60、80、100 m IU/m L)处理卵泡颗粒细胞。结果显示,基因OLR1、LHCGR、SCARA5和S100A12的表达量呈现FSH浓度依赖性升高。在3倍递增的FSH浓度梯度(0、1、3、9、27、81、243、729 m IU/m L)下观察各基因表达量的变化,发现在0~243 m IU/m L的FSH浓度范围内,LHCGR基因的表达量与FSH浓度呈高度线性相关(R2=0.982)。因此,可通过检测FSH刺激条件下细胞中LHCGR基因的表达量,来评估FSH制品的生物学效价。
译  名：
Screening of follicle-stimulating hormone dose-dependent expression genes
作  者：
LIU Tian;CHEN Xiaoyu;ZHU Zhiwei;YU Fuxian;HUANG Jing;JIA Ruoxin;SHI Fangxiong;PAN Jianzhi;College of Animal Science and Technology, Nanjing Agricultural University;Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences;Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences;
关键词：
follicle-stimulating hormone;;follicular granulosa cell;;biological value;;pig
摘  要：
To develop a new and effective method for detection of follicle-stimulating hormone(FSH) bioactivity,porcine follicular granulosa cells were used as experimental materials, and expression of genes in FSH-treated group(40 m IU/m L) and blank control group was compared by transcriptome sequencing to identify FSH-regulated candidate genes. Meanwhile, real-time fluorescent quantitative polymerase chain reaction was used to validate significantly upregulated genes after FSH treatments, and the porcine follicular granulosa cells were treated further by FSH dose gradients(0, 20, 40, 60, 80 and 100 m IU/m L). The results indicated that the expressions of OLR1, LHCGR, SCARA5 and S100 A12 increased in a FSH dose-dependent manner. In addition, the expression of these four genes was determined in FSH dose gradients(0, 1, 3, 9, 27, 81, 243 and 729 m IU/m L), and the results showed that the correlation coefficient between the expression of LHCGR and dose gradient of FSH was close to 1(R2=0.982) in the FSH dose range of 0-243 m IU/m L. In summary, LHCGR expression has a linear relationship with FSH dose, which may be helpful to develop a novel method for biological value detection of FSH.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46