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当前位置: 首页 > 文章 > 陆地棉植物络合素合酶基因的鉴定与功能预测 棉花学报 2018 (3) 215-223
Position: Home > Articles > Genome-Wide Identification and Functional Prediction of Phytochelatin Synthase Gene in Upland Cotton Cotton Science 2018 (3) 215-223

陆地棉植物络合素合酶基因的鉴定与功能预测

作  者：
梅磊;李玲;肖钦之;陈进红;祝水金
关键词：
棉花;植物络合素合酶;比较基因组学;功能预测
摘  要：
【目的】重金属胁迫对植物的生长发育有不良影响,植物络合素合酶(Phytochelatin synthase,PCS)在植物主动防御金属毒害过程中起关键作用。本文旨在对陆地棉PCS基因的数量、结构、分布和特性进行研究。【方法】根据棉属陆地棉(Gossypium hirsutum,(AD)_1),以及供体种雷蒙德氏棉(G.raimondii,D_5)和亚洲棉(G.arboreum,A_2)全基因组序列信息,结合双子叶模式植物拟南芥PCS蛋白特征域结构,对陆地棉PCS基因家族成员进行全基因组鉴定,并对其进行蛋白特征鉴定、同源类别分析、基因结构预测、酶作用位点比对以及半胱氨酸(Cys,Cysteine)分布分析。[结果]陆地棉中鉴定出4个PCS基因,而在其供体种雷蒙德氏棉和亚洲棉各鉴定出2个PCS基因。3个棉属8个PCS蛋白家族成员均含有2个特有的结构域,与催化中心相关的氨基酸位点完全保守。PCS蛋白家族在进化上分属2个不同亚组,亚组Ⅰ与亚组Ⅱ在亲缘关系上分别更接近双子叶植物和线虫,2个亚组内PCS家族在基因结构、Cys分布上存在差异,其中亚组Ⅰ较亚组Ⅱ整体内含子更长,N端Cys总数和成对Cys数量更多。雷蒙德氏棉中的2个旁系同源基因外显子完整性不及亚洲棉和陆地棉。【结论】相较于亚组Ⅱ,亚组Ⅰ的棉花PCS蛋白可能具有更强的植物络合酶活性,且陆地棉及其供体种亚洲棉对重金属的耐性强于雷蒙德氏棉。本研究为进一步研究棉花PCS的功能,以及棉花耐重金属胁迫品种改良提供理论依据。
译  名：
Genome-Wide Identification and Functional Prediction of Phytochelatin Synthase Gene in Upland Cotton
作  者：
Mei Lei;Li Ling;Xiao Qinzhi;Chen Jinhong;Zhu Shuijin;College of Agriculture and Biotechnology, Zhejiang University;Department of Plant Sciences,University of Cambridge;College of Chemistry and Bioengineering, Hunan University of Science and Engineering;Shaoyang Tobacco Monopoly Bureau;
单  位：
Mei Lei%Li Ling%Xiao Qinzhi%Chen Jinhong%Zhu Shuijin%College of Agriculture and Biotechnology, Zhejiang University%Department of Plant Sciences,University of Cambridge%College of Chemistry and Bioengineering, Hunan University of Science and Engineering%Shaoyang Tobacco Monopoly Bureau
关键词：
cotton;;phytochelatin synthase;;comparative genomics;;functional prediction
摘  要：
[Objective] Heavy metal stress rise advertise effects on growth and development in plant, from which phytochelatin synthase(PCS) plays key roles to protect plant cells. This article will present studies on the gene amount, structure, distribution and features. [Method] PCS gene family in cotton are analyzed based on completely global genome sequence cotton species including Gossypium hirsutum((AD)_1), G. raimondii(D_5) and G. arboreum(A_2), for further understanding of those genes and protein family features. In this study, we conducted the analysis involving in identification on PCS family members, special protein domain comparison, polygenetic analysis, gene structure prediction and Cysteine survey. [Result] 2, 2 and 4 PCS genes were identified out inG. raimondii(D_5),G. arboreum(A_2) andG. hirsutum((AD)_1), respectively. All these 8 PCS genes had phytochelatin and phytochelatin_C domains and strictly conserved amino acid residues related to catalytic activity. Cotton PCS protein family members could be divided into 2 sub-group, and these members belongs to sub-group Ⅰ or sub-group Ⅱ are close to dicotyledon or nematode, respectively. What's more, there are some difference in both gene structure and Cys distribution between those 2 sub-groups. Less integrity of exons in PCS genes in G. raimondii, comparing to G. hirsutum and G. arboreum.[Conclusion] Comparing to sub-group Ⅱ,the PCS genes from sub-group Ⅰshould be higher catalytic activity. G. hirsutum and its donor G. arboreum probably are more heavy metal tolerant than G. raimondii. Based on the results, this research will provide some insights on further functional study.

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