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Position: Home > Articles > Cloning of the flfA Gene of Gallibacterium anatis and Analysis of Its Function Domain Journal of Henan Agricultural Sciences 2018 (3) 138-143

鸭源鸡杆菌flfA基因的克隆及功能区域分析

作  者:
王坤芃;彭志锋;刘盼盼;王继洋;王艳;王川庆;杨霞
关键词:
鸭源鸡杆菌;flfA基因;克隆;序列分析
摘  要:
为了解鸭源鸡杆菌(G.anatis)菌毛蛋白Flf A的分布情况及功能,对18株G.anatis中国分离株的flfA基因进行扩增和分子克隆,应用相关软件对其核苷酸序列及氨基酸序列进行生物信息学分析。结果显示,18株G.anatis均具有flfA基因,核苷酸同源性为70.2%~99.8%,氨基酸同源性为63.1%~98.8%,有6~8个B细胞抗原表位,且这些优势的抗原表位多位于保守序列区,表明G.anatis不同菌株间可能存在交叉免疫反应。
译  名:
Cloning of the flfA Gene of Gallibacterium anatis and Analysis of Its Function Domain
作  者:
WANG Kunpeng;PENG Zhifeng;LIU Panpan;WANG Jiyang;WANG Yan;WANG Chuanqing;YANG Xia;College of Animal Science and Veterinary Medicine,Henan Agricultural University;College of Veterinary Medicine,Henan University of Animal Husband and Economy;
关键词:
Gallibacterium anatis;;flfA gene;;Cloning;;Sequence analysis
摘  要:
The aim of this study was to understand the distribution and function of fimbriae Flf A protein of G. anatis. flfA genes were amplified from 18 G. anatis isolates,followed by molecular cloning,bioinformatics analyses of flfA nucleotide and amino acid sequences with bioinformatics tools. The results showed that the nucleotide sequences homologies of 18 G. anatis isolates were 70. 2% —99. 8% and the deduced amino acid homologies were 63. 1% —98. 8%. FlfA protein has 6—8 B cell epitopes and most of them were located in the conserved sequence region. These results indicate that different strains may show immune cross protection.

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