Position: Home > Articles > Modeling of transplatation of colorectal tumor cell in the reproductive organs of Balb/c mouse
Journal of Gansu Agricultural University
2018
(2)
1-5
结直肠癌生殖器官移植性肿瘤动物模型的建立
作 者：
陈征;刘凌云;滚双宝;王占彬;刘忠于;李生贵;王鹏飞
关键词：
转移性肿瘤;结直肠癌;纤维蛋白凝胶;生殖器官;动物模型
摘 要：
【目的】建立CT26细胞株BALB/c小鼠生殖器官移植肿瘤模型的方法.【方法】将CT26细胞株传代培养于小鼠皮下3代后取指数生长期细胞制成悬液,采用纤维蛋白凝胶移植接种和荷包缝合移植接种2种手术方法,移植固定于小鼠生殖器官皮下,观察小鼠生理状况、成瘤率、移植瘤成长形状和组织病理学变化.【结果】纤维蛋白凝胶移植接种成瘤率为72%,雄性雌性分组平均成瘤时间(13.37±3.06)d和(12.33±2.45)d,荷包缝合移植接种成瘤率为59%,雄性雌性组成瘤时间(15.66±3.77)d和(14.25±2.08)d,成瘤组织病理切片无明显差异.【结论】纤维蛋白凝胶移植成瘤组织病理特征符合肿瘤学动物模型需求且建模成功率高.
译 名：
Modeling of transplatation of colorectal tumor cell in the reproductive organs of Balb/c mouse
作 者：
CHEN Zheng;LIU Ling-yun;GUN Shuang-bao;WANG Zhan-bin;LIU Zhong-yu;LI Sheng-gui;WANG Peng-fei;College of Animal Science and Technology,Gansu Agricultural University;
关键词：
metastatic cancer;;colorectal cancer(CRC);;fibrin gel;;reproductive organs;;animal model
摘 要：
【Objective】To set a model of transplatation of colorectal tumor cell in the reproductive organs of Balb/c mouse with CT26 cell line.【Method】The suspension was made by the cell from the 3 rdgeneration subculture with CT26 cell line,followed by the tumor cells during exponential growth phase in the suspension being transplanted into reproductive organs subcutaneous of the mouse using fibrin gelatum inoculation and pouch suture transplantation,and then observing the physiological status,tumor formation rate,tumor growth shape and histopathological changes of the mouse.【Result】The rate of tumor formation of fibrin gelatum inoculation and pouch suture transplantation were 72%and 59%,respectively.Time of group A and B tumor are respectively 13.37 and 12.33 days.Time of group C and D tumor are respectively 15.66 and 14.25 days.The difference in tumor tissue pathological slices was not significantly.【Conclusion】The pathologic features of fibrin gel transplantation were consistent with the requirement of tumor model with high success rate.
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