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Optimization of enzyme extracting process of polysaccharides from Coix lachrymal-jobi L. stems by orthogonal design.

作者：

Li-qiang Guo;Huang LiDe;Zhang ZhaoPing;Pan TingQi;Quan-tai Wen;Zu-di Yan;Huang Suo-y

单位：

Youjiang Medical College for Nationalities, Baise, Guangxi 533000, China;Youjiang Medical College for Nationalities, Baise, Guangxi 533000, Chin

关键词：

enzyme;polysaccharides;stems;lachrymal;jobi;optima

摘要：

[Objective] To determine the optimal enzyme extracting process of polysaccharides from Coix lachrymal-jobi L. stems. [Method] The cellulase extraction process of polysaccharides from C. lachrymal-jobi stems was optimized by means of orthogonal test design. And the content of polysaccharides in extracts was determined by spectrophotometry. [Result] The wavelength of the maximum absorption was 490nm. Glucose concentration showed a good linear relationship with absorbance at a range of 0.010 to 0.100mg/ml (R=0.9993). The optimal technological conditions for the extraction of polysaccharides were as follows: pH value was 5, amount of enzyme was 6.0ml, the reaction temperature was 40℃, and enzymatic hydrolysis time was 150min. Under these conditions, the mean extraction rate of polysaccharides was 19.62% with RSD of 0.21%. [Conclusion] The enzyme extracting procedure is reasonable, economical and feasible.

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Optimization of enzyme extracting process of polysaccharides from Coix lachrymal-jobi L. stems by orthogonal design.

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