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Sequence analyses on N gene of rabies viruses isolated from Guangdong Province.

作者：

Chen Jing;Jie Yuan;Changwen Ke;Wei Gu;Huang Yuan;Min Xu;Chen ShaoQin;Jianfei Zhang;Xuan Huang;Hua Xian

单位：

School of Public Health, Sun Yat-sen University, Guangzhou 510080, China;School of Public Health, Sun Yat-sen University, Guangzhou 510080, Chin

关键词：

rabies virus;n gene;strains;collected;isolated;sequence

摘要：

[Objective] To find the dominant rabies virus strains that are epidemic in Guangdong Province and thus to better prevent and control rabies. [Method] Canine brains and salivary glands collected from several regions of Guangdong Province were detected by direct immunofluorescence assay and nested RT-PCR. The isolates were further identified by intracranial inoculation in suckling mice. The N gene was amplified by RT-PCR and the sequences were aligned with those of prevalent strains in Guangxi region and vaccine strains. [Result] Two strains were isolated from specimens collected from Guangdong Province. Their N gene sequences had a high similarity of 97.9%-99.4% to those of Guangxi strains and also had a similarity of 87.7%-88.1% to those of vaccine strains of rabies virus. [Conclusion] Apparently healthy dogs may carry rabies virus, and the prevalence of rabies virus shows obvious regional differences.

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Sequence analyses on N gene of rabies viruses isolated from Guangdong Province.

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