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当前位置: 首页 > 文章 > 鸡传染性支气管炎病毒河南流行毒株S1基因的变异分析 浙江大学学报(农业与生命科学版) 2011,37 (3) 263-274
Position: Home > Articles > Variation analysis of S1 gene of avian infectious bronchitis virus epidemicstrains isolated from chicken flocks in Henan Province Journal of Zhejiang University (Agriculture and Life Sciences) 2011,37 (3) 263-274

鸡传染性支气管炎病毒河南流行毒株S1基因的变异分析

作  者：
徐雪;赵军;王川庆;王泽霖;杨霞;周欣;迪丽拜尔·阿木提
单  位：
阿克苏职业技术学院;河南农业大学禽病研究所
关键词：
传染性支气管炎病毒;肾型;呼吸型;序列分析;S蛋白;裂解位点;遗传进化分析
摘  要：
经鸡胚接种、反转录-聚合酶链式反应(RT-PCR)、气管环培养等方法从河南不同地区发病鸡群中先后共分离、鉴定出7株鸡传染性支气管炎病毒(IBV).应用RT-PCR方法对7株病毒以及IBVH120疫苗株的S1基因全序列进行扩增,并对其进行克隆测序及序列分析.结果显示:8株IBV的S1基因全长分为4类,分别是1 632、1 6201、617和1 611 bp;序列相互之间存在多位点变异,同时存在型特异性的插入和缺失;其S蛋白裂解位点序列模式有HRRRR、RRFRR和RRSRR 3种;同源性及遗传进化分析显示Jin-13为呼吸型毒株,YI为ArkDPI型肾型毒株,HN/SG株与国内其他肾型分离株亲缘关系均较远;河南肾型分离株HN/HL、XP/1/09、XP/3、WZL与山东肾型分离株之间的同源性较高且其亲缘关系最近,而与河南HN99株的亲缘关系较远.说明河南地区存在不同的IBV流行毒株,这些流行毒株在主要保护性抗原蛋白S1基因上发生了一定的变异.
译  名：
Variation analysis of S1 gene of avian infectious bronchitis virus epidemicstrains isolated from chicken flocks in Henan Province
作  者：
XU Xue1,ZHAO Jun1,WANG Chuan-qing1,WANG Ze-lin1,YANG Xia1,ZHOU Xin1,DILIBAIER Amuti2(1.Institute of poultry Diseases,Henan Agricultural University,Zhengzhou 450002,China;2.AKs Vocational and Technical College,AKs,Xinjiang 843000,China)
关键词：
infectious bronchitis virus;nephropathogenic-type;respiratory type;sequence analysis;S protein;cleavage site;phylogenetic analysis
摘  要：
Seven isolates of infectious bronchitis virus(IBV) were obtained and identified from clinical infected chicken flocks in Henan Province by using the techniques of chicken embryo inoculation,reverse transcription polymerase chain reaction(RT-PCR) and tracheal organ culture(TOC).Spike protein gene of 7 isolates and vaccine strain H120 was amplified by RT-PCR and sequenced.The result showed that the sizes of the whole length for S1gene of 8 IBV strains were divided into four groups:1 632,1 620,1 617 and 1 611 bp,respectively.There were multisite mutations among these sequences in addition to type-specific insertion and deletion.The analysis of deduced amino acid sequences showed that sequential patterns of S protein cleavage sites of 8 IBV strains fell into three categories: HRRRR,RRFRR and RRSRR.Homology and phylogenetic analysis showed that Jin-13 isolate was respiratory-type of IBV,and YI isolate was ArkDPI-type,and HN/SG isolate had a distant relationship to other domestic nephropathogenic-type isolates else,and HN/HL,XP/1/09,XP/3 and WZL isolates had a close relationship to Shandong nephropathogenic-type isolates but had a distant relationship to HN99 isolate.All results above indicate that there are different IBV epidemic isolates in Henan Province,and certain mutations at the molecular level have occurred in S1gene.

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