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当前位置: 首页 > 文章 > 杂色鲍与盘鲍种间杂交受精率的影响因素 中国水产科学 2006,13 (2) 230-236
Position: Home > Articles > Influence factors on fertilization rate in laboratory hybridization between Haliotis diversicolor and H.discus discus Journal of Fishery Sciences of China 2006,13 (2) 230-236

杂色鲍与盘鲍种间杂交受精率的影响因素

作  者：
蔡明夷;柯才焕;王桂忠;王志勇;王艺磊
单  位：
厦门大学海洋系海洋环境科学教育部重点实验室;集美大学水产生物技术研究所
关键词：
杂色鲍;盘鲍;种间杂交;受精率
摘  要：
研究卵子排放后的时间、精子排放后的时间、授精精子浓度、胰蛋白酶、钙离子、pH和水温等因子对杂色鲍(Haiotisdiversicolor,以下简称S)与盘鲍(H.discus discu,以下简称J)种间杂交受精率的影响。结果表明,卵子排放后的时间和授精精子浓度是影响杂交受精率两个最重要的因素。与自繁组合相比,正反杂交受精率均较低且下降速度快得多。水温21.5℃时,两亲本自繁对照组中,卵子排放1 h对受精率均无显著影响。而SJ杂交,卵子排放时间2 min时,受精率为55.9%±8.1%,4.7 min时受精率已下降了50%;JS杂交,卵子排放时间为4 min时,受精率为31.5%±9.7%,10 min时受精率下降至4 min时的29.1%。相比之下,精子排放时间对杂交受精率的影响小得多,3 h内对正反交杂交受精率没有显著影响。杂交适宜精子浓度约为母本自繁的100倍,其中SJ杂交适宜授精精子浓度为6.0×106~2.4×107mL-1,JS杂交适宜授精精子浓度为3.8×107~7.5×107mL-1。水温对于杂交受精率也有影响,短时间内将水温控制在26~29℃可以得到较高受精率。其他实验因素,如添加胰蛋白酶、Ca2+或改变授精体系pH对杂交受精率均无正向作用。
译  名：
Influence factors on fertilization rate in laboratory hybridization between Haliotis diversicolor and H.discus discus
作  者：
CAI Ming-yi~(1,2),KE Cai-huan~(1),WANG Gui-zhong~(1),WANG Zhi-yong~(2),WANG Yi-lei~(2)(1.Department of Oceanography,Key Laboratory of Marine Envirenmental Science,Ministry of Education,Xiamen University,Xiamen 361005,China;2.Institute of Aquaculture Biotechnology,Jimei University,Xiamen 361021,China)
关键词：
Haliotis diversicolor;Haliotis discus discus;interspecific hybridization;fertilization rate
摘  要：
To overcome the gametic incompatibility of interspecific hybridization between Haliotis diversicolor(S) and H.discus discus(J),the effects of gametic age,insemination sperm concentrations,Trypsin,Ca~(2+),pH and temperatures on the fertilization rate were investigated.The results showed that the age of egg and insemination sperm concentration are two important factors influencing the fertilization rate.In comparinson with the pure cross,the fertilization rate was low and declined much sharply for the heterospecific cross.At 21.5℃,the fertilization rate was 100% and did not declined significantly in 1 h post egg spawned for the pure cross,while it was 55.9%±8.1% at 2 min and declined by 50% at 4.7 min post spawned for the SJ cross,and it was 31.5%±9.7% at 4 min and declined by 70.9% at 10 min post spawn for the JS cross.The concentrations of sperms yielded maximum fertilization with the least abnormality in subsequent development was 6.0×10~(6)-2.4×10~(7)mL~(-1) for the SJ cross,and 3.8×10~(7)-7.5×10~(7)mL~(-1) for the reciprocal cross,which were about 100-fold higher than that in homologous cross.Higher temperature accelerated the egg losing the ability of being activated by the heterospecific sperm.For the eggs spawned for less than 10 min,the optimal temperature for hybridization is 26-(29℃).The other tested factors,including the addition of Trypsin or Ca~(2+) into the fertilization system and alteration of pH,led to decline of fertilization rate.

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