当前位置: 首页 > 文章 > 小鼠-猪种间体细胞克隆试验 动物医学进展 2010,31 (1) 31-34
Position: Home > Articles > Experiment on Somatic Cell Clone in Mouse-pig Interspecies Progress in Veterinary Medicine 2010,31 (1) 31-34

小鼠-猪种间体细胞克隆试验

作  者:
王利勤;郭慧利;芮荣
单  位:
南京农业大学动物医学院
关键词:
小鼠;猪;胎儿成纤维细胞;卵母细胞;种间核移植
摘  要:
用3代~5代小鼠胎儿成纤维细胞(MEF)作核供体细胞,体外成熟培养44 h~46 h、排出第1极体(PbI)的猪卵母细胞去核后,进行小鼠-猪种间核移植操作。Hoechst 33342染色检查去核效果,去核率为84.2%(27/32)。共培养68枚小鼠-猪种间核移植重构胚,其中48枚发生卵裂,卵裂率为70.6%,13枚胚胎发育至8-细胞,发育率达27.1%(13/48)。
译  名:
Experiment on Somatic Cell Clone in Mouse-pig Interspecies
作  者:
WANG Li-qin,GUO Hui-li,RUI Rong(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing,Jiangsu,210095,China)
关键词:
mouse;pig;embryonic fibroblast;oocyte;interspecies nuclear transfer
摘  要:
Mouse embryonic fibroblasts at 3-5 passages were used as nuclear donor cells and porcine oocytes maturated in vitro for 44-46 h with pbI were used as recipients after enucleation.Mouse-pig interspecies nuclear transfer was conducted in the present experiment.The enucleation was examined by Hoechst 33342 staining,resulting in an enucleation rate of 84.2%(27/32).A total of 68 reconstructed embryos derived from mouse-pig interspecies nuclear transfer were cultured in vitro and 48 embryos among which cleavaged,resulting in 70.6% of cleavage rate.13 reconstructed embryos developed into 8-cell stage and the developmental rate reached 27.1%(13/48).

相似文章

计量
文章访问数: 5
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊