Position: Home > Articles > Experimental Conditions for High-Speed Countercurrent Chromatography Separation of Ethanol Extract from Armillaria mellea Fermentation Broth Supernatant
FOOD SCIENCE
2012,33
(21)
245-248
高速逆流色谱法分离蜜环菌发酵上清液乙醇提取物条件的研究
作 者：
蔡丹;刘景圣;郭丽丽;刘义军;陈春阳
单 位：
吉林农业大学食品科学与工程学院
关键词：
蜜环菌发酵液;高速逆流色谱法;溶剂体系
摘 要：
利用高速逆流色谱法分离蜜环菌发酵液乙醇提取部位,筛选其最佳溶剂体系分离条件。结果表明:溶剂体系为正丁醇-乙酸乙酯-水、且体积比为1:4:5的条件下,能得到最佳分离效果,最终分离出4个峰,效果较好,4个峰的提取率分别为51.10%、1.47%、0.86%、1.80%。
译 名：
Experimental Conditions for High-Speed Countercurrent Chromatography Separation of Ethanol Extract from Armillaria mellea Fermentation Broth Supernatant
作 者：
CAI Dan,LIU Jing-sheng,GUO Li-li,LIU Yi-jun,CHEN Chun-yang(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)
关键词：
Armillaria mellea fermentation broth supernatant;high-speed countercurrent chromatography;solvent system
摘 要：
This study was designed to investigate experimental conditions for high-speed countercurrent chromatography(HSCCC) separation of the ethanol extract of Armillaria mellea fermentation broth supernatant.The best separation results were achieved using a solvent system composed of butanol,ethyl acetate and deionized water(1:4:5,V/V).Four peaks were separated well with an extraction yield of 51.10%,1.47%,0.86% and 1.80%,respectively.
相似文章
-
高效液相色谱法检测蜜环菌发酵液中腺苷含量 [蔡丹, 刘景圣, 修琳, 孔繁惠, 于中玉] 食品科学 2012,33 (20) 274-276
