Position: Home > Articles > Determination of Adenosine Content in Armillaria mellea Fermentation Broth by HPLC
FOOD SCIENCE
2012,33
(20)
274-276
高效液相色谱法检测蜜环菌发酵液中腺苷含量
作 者：
蔡丹;刘景圣;修琳;孔繁惠;于中玉
单 位：
吉林农业大学食品科学与工程学院
关键词：
蜜环菌发酵液;腺苷;高效液相色谱法
摘 要：
采用高效液相色谱法对蜜环菌发酵液中腺苷含量进行检测。色谱柱:Agilent Zorbax Eclipse XDB-C18柱(4.6mm×150mm,5μm);流动相:乙腈-水(10:90,V/V);检测波长259nm、柱温30℃、流速0.5mL/min。结果表明:线性范围为0.05～0.25mg/mL相关系数r为0.99964,精密度RSD为1.26%(n=5),稳定性RSD为1.29%(n=7),重现性实验RSD为1.76%(n=5),平均回收率99.82%。该方法准确、可靠,可以快速准确的对蜜环菌发酵液中的腺苷进行定性定量分析。
译 名：
Determination of Adenosine Content in Armillaria mellea Fermentation Broth by HPLC
作 者：
CAI Dan,LIU Jing-sheng*,XIU Lin,KONG Fan-hui,YU Zhong-yu(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)
关键词：
Armillaria mellea fermentation broth;adenosine;high performance liquid chromatography(HPLC)
摘 要：
An HPLC method was presented to determine adenosine content in Armillaria mellea fermentation broth.The chromatographic separation was performed on an Agilent Zorbax Eclipse XDB C18(4.6 mm × 150 mm,5 μm) column using a mobile phase consisting of acetonitrile and deionized water(10:90,V/V) at a flow rate of 0.5 mL/min.The column temperature was 30 ℃.Adenosine was detected at 259 nm.The HPLC method showed a linear range of 0.05-0.25 mg/mL and a correlation coefficient of 0.99964.The precision(RSD) for 5 repeated determinations was 1.26%,the stability(RSD) for 7 repeated determinations was 1.29%,and the repeatability(RSD) for 5 repeated determinations analysis was 1.76%.The average recovery across three spike levels was 99.82%(n = 3).This method was accurate,reliable,rapid and suitable for analysis of adenosine in Armillaria mellea fermentation broth.
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