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Position: Home > Articles > Effect of Factors on GUS Transient Expression of Wheat Immature Embryo Transformated by Particle Bombardment Journal of Triticeae Crops 2006,26 (2) 42-45

小麦幼胚基因枪转化的影响因素研究

作  者:
夏晓晖;陈耀锋;李春莲;吕瑞华;曹团武;曹欣;赵云祥
单  位:
西北农林科技大学农学院国家小麦改良中心杨凌分中心
关键词:
小麦幼胚;基因枪;GUS基因;瞬时表达;转化效率
摘  要:
为了明确不同因素对小麦基因枪法转化效率的影响,用PDS-1000/H e型基因枪将抑制叶片衰老基因P sag 12-ip t转入小麦幼胚细胞,通过对报告基因GUS瞬时表达的检测,研究了金粉用量、质粒DNA用量及轰击次数对转化率的影响。结果表明,金粉用量在100~400μg范围内,对幼胚瞬时表达率影响不大,幼胚瞬时表达率在73.7%~85.0%之间,但随金粉用量的增加,细胞瞬时表达率明显提高;质粒DNA用量以每枪2.0μg为最佳;轰击2枪幼胚和细胞瞬时表达率明显高于轰击1枪。
译  名:
Effect of Factors on GUS Transient Expression of Wheat Immature Embryo Transformated by Particle Bombardment
作  者:
XIA Xiao-hui,CHEN Yao-feng,LI Chun-lian,LV Rui-hua,CAO Tuan-wu,CAO Xin,ZHAO Yun-xiang(College of Agronomy,Northwest A & F University,Yangling Branch of National Wheat Improvement Center,Yangling,Shaanxi 712100,China)
关键词:
Immature embryos of wheat(Triticum aestivum L.);Particle bombardment;GUS gene;Transient expression;Transformation efficiency
摘  要:
Psag12-ipt gene was transformed into wheat immature embryo cells by particle bombardment.The effects of gold particle concentration and DNA amount per bombardment on transformation efficiency of wheat immature embryo cells were studied by GUS transient expression.The result showed that the gold particle concentration had little affect on transient expression of immature embryos,while it played a remarkable role in the cell transient expression at a relatively high gold particle amount per bombardment.It was found that the transformation frequency of wheat immature embryo cells was the superior when coated by 2.0 μg DNA and bombarded for 2 times per dish.

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