Protective effects of cytochalasin H on MPP~+-induced apoptosis in PC12 cells

YAN Yuwen;WEI Zhongqi;JU Jingjing;CHEN Qin;LIU Xiaoyang;SHEN Zhiping;SHEN Li;GE Xiaoqun;Coll of Med,Yangzhou Univ;Taizhou Med Hi-Tech Zone Admin Comm;

cytochalasin H;;PC12;;apoptosis;;MPP+;;ROS;;Parkinson's disease

To investigate the protective effects of cytochalasin H(CyH),which was a solid-fermented product of endophyte Phomopsis sp.in Vatica mangachapoi,on MPP+-induced apoptosis in PC12.PD model in PC12cells was prepared by 500μmol·L-1 MPP+.Cell viability was determined by MTT colorimetric method.LDH release was measured by colorimetric determination.Apoptotic morphology was observed by AO/EB staining method and transmission electron microscopy(TEM).Cell apoptosis rate and ROS contents were determined by Flow Cytometer.The results were as follows:1.All could significantly increase the survival rate of PC12cells and decrease LDH release rate,when CyH was delivered simultaneously(0.05—0.20μmol·L-1),before(0.002 5—0.010 0μmol·L-1)and after(0.01—0.20μmol·L-1)with MPP+.2.Flow Cytometer results showed that CyH could reduced MPP+-induced PC12cell apoptosis(P<0.001).TEM and AO/EB staining results showed that CyH could improve cell morphology.3.CyH could significantly inhibit the increase of ROS contents induced by MPP+(P<0.05).All the results show that CyH can inhibit MPP+-induced damage and apoptosis in PC12cells,which may be related to reduction of the ROS contents in cells.