Protective effects of cytochalasin H on MPP^+-induced apoptosis in PC12 cells

cytochalasin H%PC12%apoptosis%MPP^+% ROS%Parkinson's disease

To investigate the protective effects of cytochalasin H (CyH), which was a solid-fermented product of endophyte Phomopsis sp. in Vatica mangachapoi, on MPP^+-induced apoptosis in PC12. PD model in PC12 cells was prepared by 500 μmol ·L^-1 MPP^+. Cell viability was determined by MTT colorimetric method. LDH release was measured by colorimetric determination. Apoptotic morphology was observed by AO/EB staining method and transmis- sion electron microscopy (TEM). Cell apoptosis rate and ROS contents were determined by Flow Cytometer. The results were as follows: 1. All could significantly increase the survival rate of PC12 cells and decrease LDH release rate, when CyH was delivered simultaneously (0.05--0.20 μmol ·L^-1 ), before (0. 002 5--0. 010 0μmol ·L^-1 ) and after (0.01- 0.20 μmol ·L^-1) with MPP^+. 2. Flow Cytometer results showed that CyH could reduced MPP^+-induced PC12 cell apoptosis(P〉0. 001). TEM and AO/EB staining results showed that CyH could improve cell morphology. 3. CyH could significantly inhibit the increase of ROS contents induced by MPP^+ (P〈0.05). All the results show that CyH can inhibit MPP^+-induced damage and apoptosis in PC12 cells, which may be related to reduction of the ROS contents in cells.