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当前位置: 首页 > 文章 > 水牛巴贝斯虫病可溶性抗原疫苗的疫区应用试验 华中农业大学学报 2000,19 (4) 55-57
Position: Home > Articles > Field Trial of Cell Culture derived Babesia orientalis Vaccine Against Buffalo Babsiosis Journal of Huazhong Agricultural University 2000,19 (4) 55-57

水牛巴贝斯虫病可溶性抗原疫苗的疫区应用试验

作  者：
赵俊龙;刘恩勇;石俊华;丁书田;刘钟灵;姚宝安;赵胜明;刘朝华;罗永香
单  位：
华中农业大学!,武汉430070,湖北省动物血防站!武汉430064,湖北省大冶市畜牧局!大冶435160,湖北省黄石市动物检疫站!黄石435000,华中农业大学!武汉430070,华中农业大学!武汉430070,湖北省大冶市畜牧局!大冶435160,湖北省大冶市畜牧局!大冶435160,湖北省大冶市畜牧局!大冶435160;华中农业大学
关键词：
东方巴贝斯虫;可溶性抗原;免疫预防;水牛
摘  要：
体外培养获得的东方巴贝斯虫可溶性抗原与等量弗氏完全佐剂混合后用作疫苗进行疫区免疫保护试验。试验动物共分 2组 ,免疫组 82头牛在发病季节前 1个月用疫苗间隔 2周免疫 2次 ,对照组不作处理。所有试验动物在试验前和发病季节后分别采血检测血清抗体水平 ,同时 ,在发病季节观察试验动物的发病情况。结果表明 :在发病季节内 ,免疫组牛无一发病 ,血清抗体水平与试验前相比升高明显 ,而对照组 86头牛有 5头出现临床症状 ,虽经治疗仍有 2头死亡 ,血清抗体水平与试验前相比变化不明显。结果说明由可溶性抗原制备的疫苗具有良好的免疫保护作用
译  名：
Field Trial of Cell Culture derived Babesia orientalis Vaccine Against Buffalo Babsiosis
作  者：
Zhao Junlong 1) Liu Enyong 2) Shi Junhua 3) Ding Shutian 4) Liu Zhongling 1) Yao Baoan 1) Zhao Sengming 3) Liu Chaohua 3) Luo Yongxiang 2) ( 1) Huazhong Agricultural University, Wuhan 430070,China;
关键词：
Babesia orientalis , soluble antigen, immunoprophyaxis, buffalo
摘  要：
In Babesia orientalis endemic areas, cell culture derived soluble Babesia orientalis vaccine admixed with a Freund's complete adjuvant was administered in 2 dose, 2 weeks between each dose, to 82 buffaloes one month before endemic period. An additional 86 buffaloes served as nonvaccinated controls. During endemic period, clinical signs of all buffaloes were monitored, Ab titres were detected at the beginning and end of the experiment. The results show as follows: no buffaloes showed any clinical signs and no vaccinated buffaloes died during endemic period, whereas 5 of the control buffaloes showed clinical signs and 2 died. The mean Ab titres of vaccinated buffaloes after endemic period were higher than that before vaccination, whereas the mean Ab titres of control animals showed no remarkable changes before and after endemic period. These results showed that this vaccine could provide good protection against babesiosis in endemic areas.\;

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