中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 东方巴贝斯虫的18S rRNA基因的扩增及系统发育研究 畜牧兽医学报 2005,36 (9) 923-926
Position: Home > Articles > Amplification of 18S rRNA Gene of Babesia orientalis and Study on Its Phylogenesis Chinese Journal of Animal and Veterinary Sciences 2005,36 (9) 923-926

东方巴贝斯虫的18S rRNA基因的扩增及系统发育研究

作  者：
刘琴;付媛;周艳琴;周丹娜;江涛;王金苹;赵俊龙;姚宝安
单  位：
华中农业大学农业微生物国家重点实验室;华中农业大学动物医学院
关键词：
东方巴贝斯虫;水牛;PCR;18SrRNA;系统发育树;新种
摘  要：
利用真核生物18S rRNA基因的PCR通用引物对寄生于中国水牛的巴贝斯虫(已命名为东方巴贝斯虫-Babesia orientalis)基因组DNA进行扩增,得到其18S rRNA全基因片段,测序后blast分析表明该虫种属巴贝斯虫无疑。将该基因1 700 bp长片段序列与GenBank中15种已知巴贝斯虫的相应序列进行比较分析,建立系统发育树。结果表明,东方巴贝斯虫与南非未定种的巴贝斯虫亲缘关系最近,与羊巴贝斯虫亲缘关系较近,与牛巴贝斯虫、双芽巴贝斯虫的亲缘关系较远。这一结果说明水牛东方巴贝斯虫是一独立种。
译  名：
Amplification of 18S rRNA Gene of Babesia orientalis and Study on Its Phylogenesis
作  者：
LIU Qin~(1,2), FU Yuan~(1,2), ZHOU Yan-qin~2, ZHOU Dan-na~(1,2), JIANG Tao~(1,2), WANG Jin-ping~(1,2), ZHAO Jun-long~(1,2*), YAO Bao-an~2 (1. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; 2.College of Animal and Veterinary Science, Huazhong Agricultural University, Wuhan 430070,China)
关键词：
Babesia orientalis; buffalo; PCR; 18S rRNA; phylogenetic tree;new species
摘  要：
The complete 18S rRNA gene of Babesia orientalis parasited in buffalo in China was obtain by PCR. It was sequenced and then blasted. The result indicated that the parasite belonged to the genus Babesia .The 1 700 bp complete gene sequence was compared with 15 other Babesia sp. availabled in GenBank. The data were analyzed and a phylogenetic tree was established. The result indicated that the parasite was close to Babesia sp. of South Africa and B.ovis, and the distant was far to B.bigemina and B.bovis. This result indicated that it was no doubtful that it was a new species. It proved that the Buffalo babiesiosis was not mixinfected by B.bigemina and B.bovis .

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46