中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 食品中苏丹红HPLC检测方法研究 安徽农业科学 2009,37 (35) 17301-17303+17317
Position: Home > Articles > Research on High Performance Liquid Chromatograph (HPLC) for the Determination of Sudan Dyes in Food Journal of Anhui Agricultural Sciences 2009,37 (35) 17301-17303+17317

食品中苏丹红HPLC检测方法研究

作  者：
张明洲;傅小伟;刘炼;俞晓平
单  位：
中国计量学院生物安全与食品科学研究所
关键词：
苏丹红;高效液相色谱;测定;食品
摘  要：
[目的]建立食品中简便、易行检测苏丹红Ⅰ～Ⅳ的高效液相色谱方法。[方法]采用液相萃取样品处理,C18色谱柱,流动相A为1%冰醋酸的水溶液,流动相B为1%冰醋酸、20%丙酮的乙腈溶液,梯度洗脱,检测波长478 nm,对辣椒食品中的苏丹红Ⅰ～Ⅳ号进行了分离和检测。[结果]苏丹红Ⅰ～Ⅳ的标准曲线呈良好的线性关系,相关系数分别为0.999 9、0.996 6、0.997 4、0.993 7,精密度相对标准偏差分别为4.25%、5.34%、1.99%、7.56%;辣椒粉样品中的苏丹红Ⅰ～Ⅳ号的加样回收率分别为89.87%、90.90%、87.55%、81.07%;辣椒油样品中的苏丹红Ⅰ～Ⅳ号的加样回收率分别为94.83%、91.99%、88.83%和86.86%;苏丹红Ⅰ～Ⅳ号的最低检测限分别为5.02、2.46、2.30、5.02μg/kg。[结论]该方法样品处理简单、快速,灵敏度、精密度符合要求,可在基层实验室推广应用。
译  名：
Research on High Performance Liquid Chromatograph (HPLC) for the Determination of Sudan Dyes in Food
作  者：
ZHANG Ming-zhou et al (Institute of Bio-safety and Food Science,China Jiliang University,Hangzhou,Zhejiang 310018)
关键词：
Sudan dyes;High performance liquid chromatography(HPLC);Determination;Food
摘  要：
[Objective] The study aimed to establish one simple and fast analytical method for detecting Sudan Ⅰ,Ⅱ,Ⅲ,and Ⅳ in foodstuffs by high performance liquid chromatography(HPLC).[Method] The Sudan dyes were separated and detected on Microsorb-MV100 C18 column with gradient elution,using the solution of 1% acetic acid-acetonitrile of 1% acetic acid and 20% acetone as mobile phase,while the wavelength was 478 nm.[Result] The calibration curves of Sudan Ⅰ-Ⅳ showed good linearity with the correlation coefficients of 0.999 9,0.996 6,0.997 4 and 0.993 7,RSD of precision of 4.25%,5.34%,1.99% and 7.56%,respectively.The recoveries of spiked paprika samples were 89.87%,90.90%,87.55% and 81.07%,and the relative standard deviation(RSD) were 4.05%,6.58%,6.73% and 4.94%,respectively.For spiked chilli oil samples,the recoveries were 94.83%,91.99%,88.83% and 86.86% with the RSD of 6.98%,3.36%,6.71% and 3.42%,respectively.The detection limits of Sudan Ⅰ-Ⅳ were 5.02,2.46,2.30 and 5.02 μg/kg,respectively.[Conclusion] This method was simple,rapid,accurate,reliable and applicable for routine analysis.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46