当前位置: 首页 > 文章 > 高效液相色谱法快速检测食品中苏丹红的含量 安徽农业科学 2014 (5) 1532-1533+1545
Position: Home > Articles > High Performance Liquid Chromatographic Method for Rapid Determination of Sudan in Food Journal of Anhui Agricultural Sciences 2014 (5) 1532-1533+1545

高效液相色谱法快速检测食品中苏丹红的含量

作  者:
曹丽芬;姚黎霞;何良兴;茹巧美
单  位:
浙江省杭州市余杭区农产品监测中心
关键词:
高效液相色谱;苏丹红;食品
摘  要:
[目的]建立快速检测食品中苏丹红(苏丹红I、Ⅱ、Ⅲ、IV)含量的高效液相色谱法,简化样品的前处理,降低成本,缩短仪器检测时间。[方法]选用Athena C18-XDB色谱柱(4.6 mm×150.0 mm,3.5μm),以乙腈水溶液和乙腈丙酮溶液为流动相进行等度洗脱,通过二极管阵列检测器检测食品中苏丹红。[结果]该方法检测线性良好,相关系数R≥0.999 1,检出限为0.01 mg/L,相对标准偏差(n=10)为0.9%~2.0%,回收率为90%~96%。[结论]该法具有较高的准确度和精密度,适用于食品中苏丹红含量的测定。
译  名:
High Performance Liquid Chromatographic Method for Rapid Determination of Sudan in Food
作  者:
CAO Li-fen;Agricultural Products Monitoring Center in Yuhang District of Hangzhou City;
关键词:
High performance liquid chromatography(HPLC);;Sudan;;Food
摘  要:
[Objective]A analytical method was established to determine Sudan( Sudan I,Ⅱ,Ⅲ,IV) content in food by high performance liquid chromatography( HPLC) with simplified sample pre-treatment,reduced cost and shorten instrument detection time. [Method] The Sudan were separated on an Athena C18-XDB column( 4. 6 mm × 150. 0 mm,3. 5 μm) by isocratic elution using acetonitrile water solution and acetonitrile acetone solution as mobile phase,and analyzed with a diode-array detector. [Result]The method showed a good linear relationship with correlation coefficient of more than 0. 999 1. The limit of detection( LOD) was 0. 01 mg / L,the relative standard deviation( n = 10) was between 0. 9%- 2. 0%,and the recovery rate was between 90%- 96%. [Conclusion]The method has a high accuracy and precision,which is suitable for analyzing the Sudan content in food.

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