当前位置: 首页 > 文章 > 线粒体COI基因在同翅目蚜科昆虫种类鉴定中的应用研究 青岛农业大学学报(自然科学版) 2012,29 (4) 261-266
Position: Home > Articles > Study on Application of COI Gene in the Homoptera Aphididae Journal of Qingdao Agricultural University(Natural Science) 2012,29 (4) 261-266

线粒体COI基因在同翅目蚜科昆虫种类鉴定中的应用研究

作  者:
陈占秀;顾耘
单  位:
青岛农业大学农学与植物保护学院
关键词:
COI基因;蚜总科;系统发育关系;遗传;变异
摘  要:
作者于2012年在青岛农业大学昆虫实验室内,采用MtDNA中的COI基因序列,对昆虫蚜科瘤蚜属中苹果瘤蚜Myzu smalisuctus Matsumura和桃蚜Myzus persicae (Sulzer),蚜属中的棉蚜Aphis gossypii Glover、花生蚜Aphis laburni Kahenbach、洋槐蚜Aphis robiniae Macchiati、和绣线菊蚜Aphis citricola Van der Goot的无翅和有翅型,缢管蚜属中的玉米蚜Rhopalosiphum maidis (Fitch)、禾缢管蚜Rhopalosiphum padi(Linn.),以及长管蚜属中的月季长管蚜Macrosiphum roswomrn Zhang、小麦长管蚜Macrosiphum avenae (Fabricius)等10个物种的11个类型进行了系统学研究.研究结果显示,2195引物仅能对蚜属和瘤蚜属昆虫进行片段扩增,其片段长度为781bp,依此获得的系统发育关系与传统分类学完全相符.YC-1引物可用于长管蚜属和缢管蚜属中系统学研究,其片段长度为344bp,所反映的系统发育关系与传统分类学相吻合.
译  名:
Study on Application of COI Gene in the Homoptera Aphididae
摘  要:
The author researched phylogenetic relationship of ten kinds of insects(11 kinds of biological type),by the MtDNA of COI gene sequence,including Myzus(Myzu smalisuctus Matsumura,Myzus persicae(Sulzer)),Aphis(Aphis gossypii Glover,Aphis laburni Kaltenbach,Aphis robiniae Macchiati,Aphis citricola Van der Goot),Rhopalosiphum(Rhopalosiphum maidis(Fitch),Rhopalosiphum padi(Linn.)),Macrosiphum(Macrosiphum roswomm Zhang,Macrosiphum avenae(Fabricius)) in Qingdao agricultural university insects laboratory in 2012.The results showed that 2195 primer can only to aphid Myzu and Aphis insects.The fragment length is 781 bp.System development relations based on the fragment is completely consistent to the traditional taxonomy.YC-1 prime can divice insect of Rhopalosiphum and Macrosiphum.The fragment length is 433 bp.System dev1elopment relations based on the fragment is completely consistent to the traditional taxonomy.

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