当前位置: 首页 > 文章 > 不同预处理方法对菜籽蛋白酶解效果的影响 浙江农业学报 2012,24 (5) 909-913
Position: Home > Articles > Effects of different pretreatment methods on the enzymatic hydrolysis of rapeseed protein Acta Agriculturae Zhejiangensis 2012,24 (5) 909-913

不同预处理方法对菜籽蛋白酶解效果的影响

作  者:
王微星;陈剑兵;杨颖;郑美瑜;陆胜民
单  位:
浙江省农业科学院食品科学研究所;浙江师范大学化学与生命科学学院
关键词:
酶解液;蛋白酶;预处理;菜籽蛋白;肽质量分数;DPPH自由基清除率
摘  要:
以不同加热温度、超声波功率、三磷酸钠和琥珀酰酐对浓度为40 mg.mL-1菜籽蛋白液进行预处理,将处理后的菜籽蛋白液用碱性蛋白酶酶解3 h,通过比较酶解液中肽的质量分数和DPPH自由基清除率,得出结论为:在4种酶前预处理中,酶解效果最佳条件为温度60℃、超声功率500 W、三磷酸钠添加量为蛋白质量3倍和琥珀酰酐添加量为蛋白质量0.05倍,其肽质量分数分别为(35.00±0.18)%,(41.48±0.66)%,(39.27±0.74)%和(40.39±0.34)%;4种改性菜籽蛋白酶解液的DPPH自由基清除率效果均比对照组的高。
译  名:
Effects of different pretreatment methods on the enzymatic hydrolysis of rapeseed protein
作  者:
WANG Wei-xing1,2,CHEN Jian-bing2,YANG Ying2,ZHENG Mei-yu2,LU Sheng-min2,(1College of Chemistry and Life Science,Zhejiang Normal University,Jinhua 321000,China;2Institute of Food Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
关键词:
enzymatic hydrolysis solution;protease;pretreatment;rapeseed protein;peptide content;DPPH radical scavenging activity
摘  要:
In this study,rapeseed protein solution(40 mg·mL-1) was pretreated with different heat(60,80 and 100℃),ultrasonic power(200,300 and 500 W),sodium triphosphate and succinic anhydride respectively before being hydrolyzed by alcalase for 3 hours.Peptide content and DPPH radical scavenging activity in hydrolysates were investigated.Results indicted that the best pretreatment alone was 60℃,500 W,sodium triphosphate with an amount of three times of protein weight,and succinic anhydride in a dosage of 0.05 times of protein weight.Under these pretreatments,peptide contents were 35.00%±0.18%,41.48%±0.66%,39.27%±0.74% and 40.39%±0.34% respectively.The DPPH radical scavenging activities of the four modified protein hydrolysis solutions were all higher than the control.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊