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当前位置: 首页 > 文章 > 偏凸山羊草fast ω-醇溶蛋白基因克隆及与WDEIA相关的IgE结合抗原表位分析 河南农业科学 2019 (4) 9-13+81
Position: Home > Articles > Cloning of Fast ω-gliadin Gene of Aegilops ventricosa and Analysis of IgE-Binding Epitopes Related to Wheat-Dependent,Exercise-Induced Anaphylaxis Journal of Henan Agricultural Sciences 2019 (4) 9-13+81

偏凸山羊草fast ω-醇溶蛋白基因克隆及与WDEIA相关的IgE结合抗原表位分析

作  者：
霍冬敖;杜旭烨;朱斌;杨凡;郭娟
单  位：
贵州师范大学
关键词：
偏凸山羊草;fastω-醇溶蛋白;IgE结合抗原决定簇;小麦依赖-运动诱导过敏反应
摘  要：
克隆偏凸山羊草中的fastω-醇溶蛋白基因,并对其序列和IgE结合抗原表位进行分析,以期了解IgE结合抗原表位在D~v和M~v基因组中的分布,并为制备单克隆抗体进行IgE结合抗原表位的定量分析奠定基础。结果表明,从偏凸山羊草中克隆获得8个fastω-醇溶蛋白基因(KY368672—KY368679),其中,3个fastω-醇溶蛋白(KY368672—KY368674)由D~v基因组编码,其余5个(KY368675—KY368679)由M~v基因组编码。序列分析发现,获得的fastω-醇溶蛋白与已发表的fastω-醇溶蛋白具有相似的序列结构,KY368672为SRQ型ω-醇溶蛋白,KY368673和KY368674为TRQ型ω-醇溶蛋白,KY368675—KY368679均为SRL型ω-醇溶蛋白。另外,在M~v基因组编码的fastω-醇溶蛋白中,发现了与α-醇溶蛋白相似的多聚谷氨酰胺序列。IgE结合抗原表位分析表明,M~v基因组编码的fastω-醇溶蛋白比D~v基因组编码的fastω-醇溶蛋含有更多的IgE结合抗原表位。
译  名：
Cloning of Fast ω-gliadin Gene of Aegilops ventricosa and Analysis of IgE-Binding Epitopes Related to Wheat-Dependent,Exercise-Induced Anaphylaxis
作  者：
HUO Dongao;DU Xuye;ZHU Bin;YANG Fan;GUO Juan;Guizhou Normal University;Institute of Horticulture,Henan Academy of Agricultural Sciences;
单  位：
HUO Dongao%DU Xuye%ZHU Bin%YANG Fan%GUO Juan%Guizhou Normal University%Institute of Horticulture,Henan Academy of Agricultural Sciences
关键词：
Aegilops ventricosa;;Fast ω-gliadin;;IgE-binding epitopes;;Wheat-dependent,exercise-induced anaphylaxis
摘  要：
The fast ω-gliadin gene of Aegilops ventricosa was cloned,and its sequence and IgE-binding epitopes were analyzed,so as to understand the distribution of IgE-binding epitopes in D~v and M~v genomes and lay the foundation for the quantification of IgE-binding epitopes by preparation of monoclonal antibody.The results indicated that eight novel fast ω-gliadin genes were cloned from Aegilops ventricosa,and the number of fast ω-gliadins were KY368672—KY368679.Three fast ω-gliadins(KY368672—KY368674) were encoded by the D~v genome,and the other five fast ω-gliadins(KY368675—KY368679) were encoded by the M~v genome.Sequence analysis showed that the sequence structures of all fast ω-gliadins were similar to other published fast ω-gliadins.KY368672 was SRQ type ω-gliadin,KY368673 and KY368674 were TRQ type ω-gliadins,and KY368675—KY368679 were SRL type ω-gliadins.The fast ω-gliadin encoded by the M~v genome had similar polyglutamine sequence to that in α-gliadin.IgE-binding epitope analysis indicated that the fast ω-gliadin encoded by the M~v genome contained more IgE-binding epitopes than those encoded by the D~v genome.

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