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当前位置: 首页 > 文章 > 依赖于JA信号的蛋白酶抑制剂介导番茄对斜纹夜蛾的抗性 福建农林大学学报(自然科学版) 2021 (5) 595-600
Position: Home > Articles > 依赖于JA信号的蛋白酶抑制剂介导番茄对斜纹夜蛾的抗性 Journal of Fujian Agriculture and Forestry University(Natural Science Edition) 2021 (5) 595-600

依赖于JA信号的蛋白酶抑制剂介导番茄对斜纹夜蛾的抗性

作  者：
王如梦;杜逸菲;高玢垣;丁元鑫;孙仲享
单  位：
福建农林大学农学院
关键词：
茉莉酸;蛋白酶抑制剂;番茄;斜纹夜蛾;
摘  要：
蛋白酶抑制剂（PI）是番茄防御虫害的重要次生代谢物,茉莉酸（JA）信号在植物抗虫中发挥重要作用.然而,PI和JA在番茄抗虫中的关系与功能尚不明确.以重要鳞翅目害虫斜纹夜蛾、野生型番茄CM、JA突变体spr8和系统素过表达35S∷PS为材料,研究PI和JA在番茄抗虫中的关系与功能.结果表明,与取食对照CM相比,取食35S∷PS的斜纹夜蛾体重增量显著下降,而取食spr8的斜纹夜蛾体重增量显著上升,表明JA信号和系统素都参与了番茄抗虫过程.虫害后CM与35S∷PS中JA合成基因lapA1和pin2表达量显著上调,而spr8中JA合成基因表达量无明显变化,表明番茄抗虫依赖于JA信号.虫害还可以诱导CM合成PI,且35S∷PS中PI含量显著高于CM,spr8中PI含量显著低于CM,说明系统素和JA信号在PI合成中起重要作用.当取食添加PI的饲料时,斜纹夜蛾体重增长率显著下降.以上结果表明番茄受到虫害后通过JA信号调控PI的合成,从而提高对斜纹夜蛾的抗性.
作  者：
WANG Rumeng;DU Yifei;GAO Binyuan;DING Yuanxin;SUN Zhongxiang;College of Agriculture, Fujian Agriculture and Forestry University;State Key Laboratory of Biocontrol, Sun Yat-sen University;
关键词：
jasmonic acid;;protease inhibitor;;tomato;;Spodoptera litura
摘  要：
Proteinase inhibitor(PI) is an important secondary metabolite in tomato for defense against insect pests. Jasmonic acid(JA) signal plays an important role in plant resistance to pests. However, the relationship and function of PI and JA in tomato resistance to pests remain unclear. The important Lepidoptera pests Spodoptera litura, wild type tomato CM, JA mutant spr8 and systemin overexpression 35S∷PS were investigated. The results showed that compared with feeding on the control CM, the weight increment of the larvae feeding on 35S∷PS decreased significantly, while the weight increment of the larvae feeding on the spr8 group increased significantly, indicating that both JA signal and systemin were involved in tomato resistance and defense against pest. The expressions of the genes required for JA synthesis, lapA1 and pin2 increased significantly after the insect feeding on CM and 35S∷PS, while no significant changes were observed in the spr8 group, indicating that the resistance of tomato to pests depends on JA signal. Moreover, PI was synthesized in CM after being attacked by S.litura, and the content of PI was significantly higher in 35S∷PS than that in CM but significantly lower in spr8, indicating that systemin and JA signal played important roles in PI synthesis. When fed with PI-added feed, the weight gain rate of S.litura significantly decreased. The above results indicated that PI depends on JA signal to mediate tomato resistance to S.litura.

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