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当前位置: 首页 > 文章 > MDRV和H9AIV共感染对B淋巴细胞增殖功能的影响 福建农业学报 2011,26 (4) 33-36
Position: Home > Articles > The Effect on Proliferation Function of B Lymphocyte After Co-infection with MDRV and H9 AIV Fujian Journal of Agricultural Sciences 2011,26 (4) 33-36

MDRV和H9AIV共感染对B淋巴细胞增殖功能的影响

作  者：
蔡羲;林锋强;王劭;陈仕龙;程晓霞;朱小丽;李兆龙;陈少莺
单  位：
福建省农业科学院畜牧兽医研究所
关键词：
番鸭呼肠孤病毒;H9亚型水禽流感病毒;共感染;B淋巴细胞;增殖功能
摘  要：
以番鸭呼肠孤病毒(MDRV)或/和H9亚型水禽流感病毒(AIV)人工感染8日龄健康雏番鸭,应用MTT法测定感染后7、14和21d番鸭血液、脾脏和法氏囊中的B淋巴细胞增殖功能的变化动态,探讨MDRV或/和H9亚型AIV对B淋巴细胞增殖功能的影响。结果显示8日龄番鸭人工感染后,MDRV感染、AIV感染和共感染组番鸭血液、法氏囊和脾脏的B淋巴细胞刺激指数均明显低于健康对照组,而且共感染组番鸭B淋巴细胞刺激指数低于MDRV感染和AIV感染组,表明MDRV和H9AIV病毒感染均能抑制番鸭外周血液及体液免疫器官(法氏囊、脾脏)中B淋巴细胞增殖功能,MDRV与H9AIV共感染加重B细胞免疫抑制程度。
译  名：
The Effect on Proliferation Function of B Lymphocyte After Co-infection with MDRV and H9 AIV
作  者：
CAI Xi,LIN Feng-qiang,WANG Shao,CHEN Shi-long,ZHU Xiao-li,CHEN Xiao-xia, LI Zhao-long,CHEN Shao-ying(1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences, Fuzhou,Fujian 350013,China;2.Fujian Animal Diseases Control Technology Development Center, Fuzhou,Fujian 350013,China)
关键词：
MDRV;H9AIV;Co-infection;B lymphocytes;Proliferation
摘  要：
To reveal the immunosuppression mechanisms,eight-day-old Muscovy ducklings,were challenged with Muscovy duck reovirus(MDRV) and avian influenza virus(H9AIV) individually and simultaneously.The changes of proliferation of B lymphocytes in the blood,spleens and bursa were determined by MTT method 7 days,14 days and 21 days after the Muscovy ducklings were challenged.The results show that B lymphocyte stimulation indexes in the blood,bursa and spleen of the challenged group were significantly lower than that in control group,and B lymphocytes stimulation index in co-infection group was the lowest.So B lymphocytes proliferation of peripheral blood and humoral immune organs(bursas,spleens) in Muscovy ducklings was inhibited by both MDRV and H9AIV,and co-infection with MDRV and H9AIV could aggravate this immunosuppression.

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