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当前位置: 首页 > 文章 > 核桃与铁核桃种间关系的RAPD鉴定 四川农业大学学报 2007,25 (4) 144-147
Position: Home > Articles > Identification of Relationship between Juglans regia L.and J.sigillata Dode by RAPD Markers Journal of Sichuan Agricultural University 2007,25 (4) 144-147

核桃与铁核桃种间关系的RAPD鉴定

作  者：
陈良华;胡庭兴;张帆
单  位：
四川农业大学林学园艺学院
关键词：
核桃;铁核桃;RAPD;基因池
摘  要：
运用RAPD技术对核桃与铁核桃特征性条带进行标记。分别利用8个核桃样品和8个铁核桃样品构建2个物种的基因池,用327个随机引物,选出10个多态性引物,进一步用这10个引物进行单株确认,发现只有引物D6在核桃类群的8个样品中扩增出一条250bp的特异带,而在铁核桃类群的8个样品中无此特异带。初步分析认为,D6-250可能是核桃与铁核桃之间的特异性分子标记,该结果可为核桃和铁核桃种质鉴定提供一定的理论依据。
译  名：
Identification of Relationship between Juglans regia L.and J.sigillata Dode by RAPD Markers
作  者：
CHEN Liang-hua,HU Ting-xing,ZHANG Fan(College of Forestry and Horticulture,Sichuan Agricultural University,Yaan 625014,Sichuan,China)
关键词：
Juglans regia L.;J.sigillata Dode;RAPD;DNA-Pool
摘  要：
Random amplified polymorphic DNA(RAPD) was applied to detect the relationship at the species level between Juglans regia L.and J.sigillata Dode.Both J.regia DNA-pool and J.sigillata DNA-pool were set up by using 8 J.regia samples and 8 J.sigillata samples,respectively.327 primers were screened,of which 10 polymorphic primers were identified.Two DNA-pools and all individuals were verified by using the 10 polymorphic primers three times.A 250 bp DNA band was amplified only from primer in D6 J.regia DNA-pool and 8 corresponding samples of J.regia,but absent in both J.sigillata DNA-pool and 8 corresponding samples of J.sigillata.The results tentatively showed that D6-250 was a special molecular marker related to J.regia and J.sigillata at the species level.This marker can provide some basis for the relationship identification of germplasm resources of the two species.

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