中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 川芎多糖的分离纯化及其单糖组成测定 四川农业大学学报 2011,29 (1) 59-63
Position: Home > Articles > Purification and Analysis of Monosaccharide Composition of Ligusticum chuanxiong Polysaccharide Journal of Sichuan Agricultural University 2011,29 (1) 59-63

川芎多糖的分离纯化及其单糖组成测定

作  者：
孙晓春;颜军;何钢;张良蕾;易勇;苟小军
单  位：
四川抗菌素工业研究所;成都大学中药化学实验室
关键词：
川芎;多糖;单糖;衍生化;高效液相色谱法
摘  要：
为了解川芎多糖的分子量和单糖组成,为药量学研究和中成药加工奠定基础,采用水提醇沉法提取川芎多糖,DEAE-纤维素柱层析法纯化,高效凝胶渗透色谱法测定分子量。然后用硫酸水解,1-苯基-3-甲基-5-吡唑啉酮衍生水解后的单糖,衍生物采用高效液相色谱法测定多糖的单糖组成。结果表明,DEAE-纤维素柱层析获得3个川芎多糖组分LCXP-1、LCXP-2和LCXP-3,分子量分别为11.916、20.793和701.875kDa。LCXP-1和LCXP-2均由甘露糖、葡萄糖、半乳糖、阿拉伯糖组成,摩尔比分别为1:495:3.7:1.2和1:632:4.3:1.2;LCXP-3由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成,摩尔比为1:6.5:1.5:248:50:14。该方法准确、灵敏度高,适用于川芎多糖中单糖组成的测定。
译  名：
Purification and Analysis of Monosaccharide Composition of Ligusticum chuanxiong Polysaccharide
作  者：
SUN Xiao-chun1,YAN Jun2,HE Gang2,3,ZHANG Liang-lei1,YI Yong2,GOU Xiao-jun2(1.Sichuan Industrial Institute of Antibiotic,Chengdu 610052,China; 2.The Laboratory of Medicinal Chemistry,Chengdu University,Chengdu 610106,China; 3.Maize Research Institute,Sichuan Agricultural University,Wenjiang 611130,Sichuan,China)
关键词：
Ligusticum chuanxiong Hort;polysaccharide;monosaccharide;derivatization;high performance liquid chromatography
摘  要：
To study the monosaccharide compositions and molecular weights of Ligusticum chuanxiong polysaccharide,and lay the foundation for pharmacometry and processing of proprietary Chinese medicine,the polysaccharide was extracted by hot water and precipitated by alcohol.The product was purified by DEAE-Cellulose,molecular weights were determined by high performance gel permeation chromatography(HPGPC) and the monosaccharide compositions were determined by HPLC using precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone(PMP).The results showed that three polysaccharides(LCXP-1,LCXP-2 and LCXP-3) were obtained,molecular weights were 11.916,20.793 and 701.875 kDa,respectively.Both LCXP-1 and LCXP-2 were composed of mannose,glucose,galactose and arabinose in molar ratio of 1∶495∶3.7∶1.2 and 1∶632∶4.3∶1.2;LCXP-3 was composed of mannose,rhamnose,galacturonic acid,glucose,galactse and arabinose in molar ratio of 1∶6.5∶1.5∶248∶50∶14.The method is accurate and sensitive,thus suitable for the analysis of monosaccharide composition of Ligusticum chuanxiong polysaccharide.

相似文章

导出引用
计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46