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Position: Home > Articles > Optimization of Prokaryotic Expression of Recombinant Caffeic Acid 3-O-methyltransferase FromLigusticum chuanxiongin E.coli Northern Horticulture 2016 (18) 107-110

川芎咖啡酸-3-O-甲基转移酶的原核表达与条件优化

作  者:
宋婧;朱建全;张泉宝;张赶;周嘉裕
单  位:
西南交通大学生命科学与工程学院
关键词:
川芎;咖啡酸-3-O-甲基转移酶;原核表达;条件优化
摘  要:
以川芎咖啡酸-3-O-甲基转移酶基因(LCCOMT)为表达对象,将含有6*His标签的LCCOMT基因连接pET28a表达载体后转化大肠杆菌BL21,采用原核表达的方法,优化了LCCOMT基因的表达条件。结果表明:当诱导时间为4h,IPTG终浓度为2.0mmol·L-1,诱导温度为37℃时,重组LCCOMT蛋白的表达量达到最大。溶解性检测结果表明,LCCOMT重组蛋白以可溶性蛋白的形式存在,占总蛋白的9%,该试验可为LCCOMT的大规模表达纯化奠定基础。
译  名:
Optimization of Prokaryotic Expression of Recombinant Caffeic Acid 3-O-methyltransferase FromLigusticum chuanxiongin E.coli
作  者:
SONG Jing;ZHU Jianquan;ZHANG Quanbao;ZHANG Gan;ZHOU Jiayu;College of Life Sciences and Engineering,Southwest Jiaotong University;
关键词:
Ligusticum chuanxiong;;caffeic acid 3-O-methyltransferase;;prokaryotic expression;;conditions optimization
摘  要:
In order to efficiently express Ligusticum chuanxiongcaffeic acid 3-O-methyltransferase(LCCOMT)in E.coli,pET28a-LCCOMT expression vector,which contained LCCOMTgene with 6*His labels,was transformed into E.coli BL21.The expression conditions of LCCOMTgene were optimized.The results showed that when the induction time was4 hours,IPTG concentration was 2.0mmol·L-1 and temperature was 37 ℃,the expression of LCCOMT recombinant protein might reach the maximum.Furthermore,solubility test results showed that the LCCOMT recombinant protein existed in the form of soluble protein.Therefore,this research laid a foundation for the future large-scale expression and purification of LCCOMT protein.

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