中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 超高效液相色谱-串联质谱法检测辅助降血脂保健食品中6种非法添加化学药物 食品科学 2018 (18) 320-325
Position: Home > Articles > Determination of Six Antilipemic Chemicals Illegally Added in Antilipemic Functional Foods by UPLC-MS/MS FOOD SCIENCE 2018 (18) 320-325

超高效液相色谱-串联质谱法检测辅助降血脂保健食品中6种非法添加化学药物

作  者：
李晓蕾;陈军;杨朝芬;杨俊
单  位：
昆明理工大学分析测试研究中心;中国林业科学研究院资源昆虫研究所
关键词：
超高效液相色谱-串联质谱法;辅助降血脂;化学药物;保健食品;非法添加
摘  要：
建立超高效液相色谱-串联质谱法同时快速检测辅助降血脂类保健食品中6种降血脂药物(烟酸、普伐他汀钠、氟伐他汀钠、美伐他汀、洛伐他汀、辛伐他汀)的方法。样品经甲醇提取,Waters Acquity BEH-C18色谱柱分离,0.1%甲酸-乙腈溶液梯度洗脱,流速0.2 m L/min,电喷雾离子源正离子模式扫描,多反应监测模式测定,外标法定量。结果表明:在实验条件下4 min内可快速、准确地定性定量分析6种降血脂化学药物,分离度良好,方法检出限为4~39μg/kg,加样回收率为80.2%~106.1%。利用本方法检测20批降血脂类保健食品,其中3批分别含有烟酸和洛伐他汀。本方法线性关系良好、快速、简便、准确,可作为辅助降血脂类保健食品中非法添加化学药物的定性定量检测方法。
译  名：
Determination of Six Antilipemic Chemicals Illegally Added in Antilipemic Functional Foods by UPLC-MS/MS
作  者：
LI Xiaolei;CHEN Jun;YANG Chaofen;YANG Jun;Research Center of Analysis and Measurement, Kunming University of Science and Technology;Research Institute of Resources Insects, Chinese Academy of Forestry;
关键词：
ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS);;antilipemic;;chemicals;;healthy foods;;illegal additives
摘  要：
A method for the simultaneous determination of six blood lipid regulating drugs(nicotinicacid, pravastatin sodium, fluvastatin sodium, mevastatin, lovastatin and simvastatin) illegally added to antilipemic functional foods by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was established. The samples were extracted with methanol. The analytes were separated on a Waters Acquity BEH-C18 column with a mobile phase consisting of 0.1% aqueous formic acid solution and acetonitrile using gradient elution at a flow rate of 0.2 m L/min, detected by multiple reaction monitoring(MRM) with an electrospray ionization source in the positive ion mode, and quantified by the external standard method. Under the experimental conditions in this study, all analytes were separated well, and they were quantified accurately and rapidly within 4 min. The limits of detection of the method ranged from 4 and 39 μg/kg, and the recoveries for spiked samples were 80.2%–106.1%. Twenty batches of antilipemic functional foods were tested by the method and out of these, 3 were found to contain nicotinic acid and lovastatin. This method exhibited good linear relationships and it was fast, simple and highly accurate, and thus could be used for the simultaneous determination of the six drugs illegally added in antilipemic functional foods.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46