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Position: Home > Articles > SNP analysis of IGFBP-5 gene exons and relationship with carcass weight in red steppes Chinese Journal of Veterinary Science 2010,30 (7) 1005-1008

草原红牛IGFBP-5基因外显子多态性及对胴体性状的影响

作  者:
芦春艳;杨润军;姜昊;张金玉;张国梁;赵玉民;赵志辉
单  位:
吉林省农业科学院畜牧分院;吉林大学畜牧兽医学院;吉林大学实验动物中心
关键词:
中国草原红牛;IGFBP-5;PCR-SSCP;PCR-RFLP;胴体性状
摘  要:
为揭示草原红牛IGFBP-5基因外显子多态性与胴体性状的相关性,采用PCR-SSCP及PCR-RFLP两种方法分别对58头草原红牛群体该基因外显子多态性进行了检测,并对检测到的突变位点用SPSS软件分析其与胴体性状的相关性。结果表明:IGFBP-5基因第3外显子在191bp处存在G(A)突变,该基因多态性与宰前活重、胴体重呈显著相关,BB型显著高于AA型(P>0.05);对其他性状的影响差异不显著。通过研究IGFBP-5基因外显子的多态性,为草原红牛的选种选育提供实验依据。
译  名:
SNP analysis of IGFBP-5 gene exons and relationship with carcass weight in red steppes
作  者:
LU Chun-yan1,YANG Run-jun1,JIANG Hao2,ZHANG Jin-yu1,ZHANG Guo-liang3,ZHAO Yu-min3,ZHAO Zhi-hui1 (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Centre of Experimental Animal,Jilin University,Changchun 130062,China;3.Branch of Animal Science,Jilin Academy of Agricultural Sciences,Gongzhuling,Jilin 136100,China)
关键词:
Chinese red steppes;IGFBP-5;PCR-SSCP;PCR-RFLP;carcass weight
摘  要:
This experiment was conducted to detecte SNP of IGFBP-5 gene exons and the relationship with carcass weight in red steppes.The polymorphism of IGFBP-5 exons in red steppes was detected by PCR-SSCP and PCR-RFLP.And then the genetic correlations between genotypes and performances was obtained by SPSS.The results indicated that there was one single nucleotide mutation G(A) in the 191 bp of exon 3 of IGFBP-5 gene;And the genetic polymorphisms significantly correlated with living weight.And living weight of BB genotype was higher than AA genotype(P<0.05).Other traits were not significantly affected.The research laid a foundation for the future studies of the biological function of IGFBP-5 gene genetic in red steppes and a theoretical basis for breeding of red steppes.

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