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Position: Home > Articles > Expression characteristic of CAPN1 gene at mRNA level in Chinese red steppe Chinese Journal of Veterinary Science 2012,32 (9) 1302-1304

草原红牛CAPN1基因mRNA表达特性分析

作  者:
秦立红;赵玉民;吴健;李旭;刘基伟;胡成华;赵志辉;张国梁
单  位:
吉林省农业科学院畜牧分院;吉林大学畜牧兽医学院
关键词:
中国草原红牛;实时荧光定量PCR;CAPN1基因
摘  要:
为了进一步揭示CAPN1的功能,本研究采用实时荧光定量PCR技术对草原红牛初生牛与成年牛心脏、肝脏、脾脏、肺脏、肾脏、瘤胃、十二指肠、背最长肌8种组织中的CAPN1mRNA表达水平进行了分析。结果表明:CAPN1基因在组织中普遍表达,但表达量存在差异。且成年牛的CAPN1基因在肺脏和瘤胃组织中的表达显著高于初生牛(P<0.05),成年牛在肝脏、脾脏、肾脏和背最长肌组织中CAPN1基因的表达水平极显著高于初生牛(P<0.01)。
译  名:
Expression characteristic of CAPN1 gene at mRNA level in Chinese red steppe
作  者:
QIN Li-hong1,ZHAO Yu-min1,WU Jian1,LI Xu1,LIU Ji-wei1,HU Cheng-hua1,ZHAO Zhi-hui2*,ZHANG Guo-liang1*(1.Branch of Animal Science,Jilin Academy of Agricultural Sciences,Gongzhuling,Jilin 136100,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China)
关键词:
Chinese Red Steppe;Real-time quantitative PCR;CAPN1
摘  要:
The present study was designed to detect the expression of CAPN1 mRNA in the heart,liver,spleen,lung,kidney,rumen,duodenum,longissimus dorsi of newborn and adult Chinese Red Steppe by Real-time RT-PCR.The results showed that CAPN1mRNA was expressed in all of eight tissues detected.We also found that CAPN1mRNA expression level was higher in lung and rumen of adult than newborn(P<0.05);but the expression level of CAPN1 mRNA expression level was significant higher in liver,spleen,kidney and longissimus dorsi of adult than newborn(P<0.01).

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