中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 苹果组织培养苗离体嫁接的研究 园艺学报 1985 (3) 151-154+2
Position: Home > Articles > STUDIES ON THE GRAFTING IN VITRO OF APPLE Acta Horticulturae Sinica 1985 (3) 151-154+2

苹果组织培养苗离体嫁接的研究

作  者：
王际轩;李淑珍;隋长琴;李博文
单  位：
辽宁省果树科学研究所
摘  要：
用MS培养基附加BA0.5ppm接种萌动前后的M_4、M_7、M_(26)、山定子、圆叶海棠等苹果砧木的腋芽生长点,在25～30℃温度下通过暗培养和光照培养,获得分化绿苗,再转接于1/2MS培养基附加ⅠAA1.0～1.5ppm的生根培养基上,长成苗高2厘米以上的生根苗,做为离体嫁接用砧木,另用MS培养基附加BA2ppm,接种富土系、金冠系和甜黄魁等苹果品种的腋芽生长点,培养分化出高1厘米以上的绿苗,做为接穗品种,进行离体嫁接。嫁接中对比了三种接法和三种接后滴抹接合部的植物激素。结果表明,采用插入接并于接合部滴抹100ppmZT或GA有较高的成活率。插入接滴ZT的成活率为40.7～82.1％;插入接滴GA的成活率为39.1～54.3％。嫁接苗继续在生根培养基上光照培养3周左右,经砂培2～3周,再转入土壤培养,成活率为26.7～56.9％。
译  名：
STUDIES ON THE GRAFTING IN VITRO OF APPLE
作  者：
Wang Jixuan Li Shuzhen Sui Changqin Li Bowen ( Pomological Institute of Liaoning province )
摘  要：
The growing apices of axillary buds of M4、 M7、 M26 of malus baccata and M.prunifolia apple rootstocks before and after germination were cul-tured in MS medium containing BA 0.5ppm. Through dark culture and light culture in 25~30℃ constant temperature, green plantlets were ob-tained. Then they were cultured in rooting medium(l/2 MS medium con-taining IAA 1.0~1.5ppm), and when they grew to more than 2 cm high, they were used as rootstocks for grafting.The growing apices of axillary buds of 'Fuji', 'Golden Delicious' and 'Tianhuangkui' apples were cultured in MS medium containing BA 2.0ppm,and obtained plant-lets of more thanlcm in height which were used as scion varities. The rootstocks and scions were grafted in vitro. Three grafting methods and three plant regulators applied on graft union were compared. The result showed that there was higher survival rate by inserting graftage with l00ppm ZT or GA applied on the graft union. . The survival rate with ZT was 40.7~82.1%, with GA 39.1~54.3%. The grafted plantlets were light-cultured continuously in rooting medium for about 3 weeks, then sand-cultured for 2~3 weeks, finally planted in soil. The survi-val rate was 26.7~56.9%。

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46