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Position: Home > Articles > EMBRYO CULTURE OF HAWTHORN ( CRATAEGUS PINNATIFIDA ) ROOTSTOCKS Acta Horticulturae Sinica 1982 (4) 7-10

山楂砧木苗的种胚培养

作  者:
王际轩;李淑珍;李博文;任恩瀛;隋长琴
单  位:
辽宁省果树科学研究所
摘  要:
把MS培养基的各种盐和蔗糖用量减半,辅加BA0.25ppm,IAA0.5ppm及可溶干酪素150ppm做成的培养基,接种近成熟或已成熟山里红种子的种胚,置25~30℃恒温和1000~1500Lux光照的培养室中培养。接种种胚的分化率为84.5%。一枚种胚经10个月的培养、增殖,获得无根绿苗1600多株。把MS培养基的各种成分的用量减半,辅加IBA1.5ppm做成生根培养基,接种3厘米以上的种胚分化苗,生根率为84 .2%。健壮的生根苗,通过砂培过渡3周左右,转至土壤培养,成活率达57.1%。这将为山楂生产快速提供砧木,解决育苗难的问题提出一条有希望的途径。
译  名:
EMBRYO CULTURE OF HAWTHORN ( CRATAEGUS PINNATIFIDA ) ROOTSTOCKS
作  者:
Wang Jixuan Li Shuzhen Li Bowen Ren Enying Sui Changqin ( The Pomological Institute of Liaoning Province )
摘  要:
Matured or near-matured embryo of Hawthorn(Crataegus pinnatifida) were cultured in the MS medium which consisted of half as much as various salts, sucrose and supplemented with BA 0.25ppm, IAA0.5ppm and casein hydrolysate 150ppm. It was placed in a culture chamber with constant temperature at 25-30℃ , under 1000-1500 Lux light intensity, Differentiated seedlings were obtained, the rate of differentiation being 84.5%. More than 1600 seedlings were obtained from one embryo in a period of ten months. In order to promote rooting, the seedlings weve transferred to MS medium of half salts and sucrose and supplemented with IBAl.5ppm. Rooted seedlings were obtained, the rooting rate being 84.2%. The rooted seedlings were grown in sand for 3 weeks, then transplanted to soil. The survival rate of the seedlings was 57.1%。

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