单 位：
北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室
关键词：
低聚葡萄籽原花青素;顺铂;细胞增殖;细胞周期
摘 要：
目的:探讨低聚葡萄籽原花青素(oligomer grape seed proanthocyanidins,O-GSP)联合顺铂(cisdichlorodiamineplatinum(Ⅱ),DDP)对A549细胞增殖、细胞周期及细胞周期相关蛋白表达的影响。方法:体外培养A549细胞,四甲基偶氮唑蓝法检测DDP和O-GSP单独及联合用药对A549细胞存活率的影响;流式细胞仪检测O-GSP联合DDP对A549细胞周期的影响;Western Blot检测促细胞周期相关蛋白CDK 4、Cyclin D1和p-Rb的表达。结果:DDP(≥5 mg/L)和O-GSP(≥16 mg/L)单独用药均对A549细胞增殖有抑制作用,且两者同时作用对细胞增殖抑制具有联合作用。5 mg/L DDP单独用药可以于S期阻滞A549细胞,4 mg/L O-GSP单独用药可以于G_0/G_1期阻滞A549细胞,两者联合可显著降低细胞周期调控蛋白CDK 4、Cyclin D1和p-Rb的表达(P<0.05)。结论:O-GSP可以联合DDP抑制A549细胞增殖,且这种联合作用与Cyclin D1-CDK 4-Rb通路介导的细胞周期调控有关。
译 名：
Effect of Oligomeric Proanthocyanidins from Grape Seeds Combined with Cisplatin on A549 Cell Proliferation and Cell Cycle
作 者：
WANG Hai;LIAN Yanna;GAO Liping;Beijing Municipal Key Laboratory of Biologically Active Substances and Functional Food, College of Arts and Science,Beijing Union University;
关键词：
oligomeric proanthocyanidins from grape seeds;;cisplatin;;cell proliferation;;cell cycle
摘 要：
Objective: To investigate the effect of oligomeric proanthocyanidins from grape seeds(O-GSP) combined with cisplatin(DDP) on the proliferation, cell cycle and cell cycle-related protein expression of A549 cells. Methods: A549 cells were cultured in vitro. The effects of DDP and O-GSP alone and in combination on the survival rate of A549 cells were observed by MTT assay; the combined effect of O-GSP and DDP on the cell cycle of A549 cells was detected by flow cytometry and the expression levels of the cell cycle-related proteins CDK 4, Cyclin D1 and p-Rb were detected by Western blot. Results: DDP(≥ 5 mg/L) and O-GSP(≥16 mg/L) treatments alone could inhibit A549 cell proliferation. The combined treatment had a synergistic effect on the inhibition of cell proliferation. DDP treatment alone at 5 mg/L arrested A549 cells at S phase and O-GSP at 4 mg/L arrested the cells at G_0/G_1 phase. The combination of DDP at 5 mg/L and O-GSP at 4 mg/L significantly reduced the expression levels of the cell cycle regulatory proteins CDK 4, Cyclin D1 and p-Rb. Conclusion: DDP can inhibit the proliferation of A549 cells in combination with O-GSP through a mechanism related to the regulation of cell cycle progression mediated by the Cyclin D1-CDK 4-Rb pathway.
