单 位：
北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室
关键词：
低聚葡萄籽原花青素;顺铂;肺腺癌;细胞凋亡
摘 要：
目的:研究低聚葡萄籽原花青素(oligomeric?grape?seed?proanthocyanidins,O-GSP)联合顺铂对人肺腺癌细胞A549抗氧化、细胞凋亡及凋亡相关蛋白表达的影响。方法:细胞分为对照组、顺铂(5 mg/L)组、O-GSP(4 mg/L)组、O-GSP(4 mg/L)+顺铂(5 mg/L)组。硫代巴比妥酸法测定细胞中丙二醛(malondialdehyde,MDA)含量;二硫代二硝基苯甲酸法测定谷胱甘肽(glutathione,GSH)含量;黄嘌呤氧化酶法测定超氧化物歧化酶(superoxide?dismutase,SOD)活力;流式细胞术检测细胞内活性氧(reactive?oxygen?species,ROS)含量和细胞凋亡率;Western?blot检测细胞凋亡相关蛋白Bcl-2、Bax和caspase-3的表达。结果:顺铂导致A549细胞MDA、ROS含量显著升高(P<0.05),GSH含量和SOD活力显著降低(P<0.05),O-GSP对细胞内MDA含量、GSH含量、SOD活力无显著影响(P>0.05)。O-GSP+顺铂组相对于顺铂组,细胞内MDA、GSH、SOD水平无显著性变化(P>0.05),而ROS含量明显降低(P<0.05)。顺铂、O-GSP以及两者联用均能显著促进细胞凋亡(P<0.05),并且能够显著提高Bax及Bax/Bcl-2值,顺铂和O-GSP+顺铂可显著降低Bcl-2的表达(P<0.05),同时激活caspase-3。结论:O-GSP联合顺铂可促进A549细胞的凋亡,其机制与O-GSP的抗氧化作用没有显著联系,而是与两者可共同调节凋亡相关蛋白基因表达有关。
译 名：
Effect of Oligomeric Grape Seed Proanthocyanidins Combined with Cisplatin on the Apoptosis of Human Lung Adenocarcinoma Cell Line A549
作 者：
WANG Hai;LIAN Yanna;ZHOU You;HUANG Shanwan;GAO Liping;Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Arts and Sciences,Beijing Union University;
关键词：
oligomeric?grape?seed?proanthocyanidin(O-GSP);;cisplatin;;lung?adenocarcinoma;;cell?apoptosis
摘 要：
Objective:?To?study?the?effect?of?oligomericgrape?seed?proanthocyanidin(O-GSP)?combined?with?cisplatin(CDDP)?on?antioxidant?defense?systems,?cell?apoptosis?and?apoptosis-related?protein?expression?in?human?lung?adenocarcinoma?cell?line?A549.?Methods: The?cells?were?divided?into?control,?CDDP(5?mg/L),?O-GSP(4?mg/L),?and?O-GSP(4?mg/L)?+?CDDP(5?mg/L)?groups.??Malondialdehyde(MDA) content was measured by thiobarbituric acid method.?Glutathione(GSH) content was?determined by nitrobenzoic acid method.?Superoxide dismutase(SOD) activity was examined by xanthine oxidase method.?Flow?cytometry?was?used?to?detect?the?intracellular?ROS?content?and?apoptosis?rate.?The?expression?of?apoptosis-related?proteins?such?as?Bcl-2,?Bax?and?caspase-3?were?detected?by?Western?blot.?Results:?CDDP?resulted?in?a?significant?increase?of?MDA?and?ROS?in?A549?cells(P??0.05).?Compared?with?the?CDDP?group,?the?levels?of?MDA,?GSH?and?SOD?in?the?O-GSP?+?CDDP?group?were?not?significantly?changed(P?>?0.05),?while?the?content?of?ROS?was?significantly?reduced(P?
