中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 不同种源印楝遗传多样性的ISSR分析 林业科技 2009,34 (1) 5-8
Position: Home > Articles > ISSR Analysis of Genetic Diversity of Neem in Different Provenance Forestry Science & Technology 2009,34 (1) 5-8

不同种源印楝遗传多样性的ISSR分析

作  者：
范刚;尹鸿翔;杜娟;张艺;唐锐;解培惠
单  位：
成都中医药大学民族医药学院;攀枝花市干热河谷应用生态研究中心;四川大学生命科学院
关键词：
印楝;种源;遗传多样性;ISSR
摘  要：
利用ISSR分子标记技术对印楝进行遗传多样性分析,用9条引物进行扩增,共检测出81条清晰的位点,其中79条具有多态性,多态位点百分率为97.53%,Ne i s基因多样性指数为0.380 3,Shannon信息指数为0.553 7,表明印楝遗传多样性很丰富。种源间遗传分化系数为0.470 2,Shannon s居群分化系数为0.467 7,表明印楝种源内的遗传分化大于种源间的分化。聚类分析将13个种源分为2大类,来自缅甸的11个种源聚成一类,来自澳大利亚和印度的种源聚成另外一类。结果表明,ISSR分子标记技术适合于印楝的遗传多样性分析。
译  名：
ISSR Analysis of Genetic Diversity of Neem in Different Provenance
作  者：
FAN Gang(Chengdu University of TCM,Sichuan Chengdu 611137)
关键词：
Azadirachta indica;Provenance;Genetic diversity;Inter-simple sequence repeat(ISSR)
摘  要：
The genetic diversity of neem was analyzed by ISSR technique.9 appropriate primers were selected for ISSR PCR amplification.The 9 primers could amplify 81 bands.Of these,79 were polymorphic loci.The results showed that high levels of genetic diversity existed in the populations(PPB was 97.53%,H was 0.380 3 and I was 0.553 7).Coefficient of gene differentiation Gst was 0.470 2,Shannon's diversity index was 0.467 7,which showed that the genetic variation of neem mainly came from intra-provenance.The cluster analysis showed that thirteen provenances were divided into two clusters.One was consisted of eleven provenances of Burma,and the other was Australia and India provenances.This fact indicated that eleven Burma provenances have a close genetic relationship,so do Australia and India provenances.The results showed that ISSR method was suitable for genetic diversity analysis of Neem,which provide the scientific basis of developing effective protection measures for these species.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46