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Position: Home > Articles > Development of ISSR Markers and Analysis of Genetic Diversity of Asimina triloba Forest Science and Technology 2018 (11) 3-6

北美泡泡树ISSR标记开发及遗传多样性分析

作  者:
有祥亮;罗玉兰;尹丽娟;张爱明;张冬梅
单  位:
上海城市困难立地绿化工程技术研究中心;上海市园林科学规划研究院;国家林木种质资源平台-上海子平台
关键词:
泡泡树;Asimina triloba;ISSR标记;遗传多样性;聚类分析
摘  要:
以泡泡树[Asimina triloba (L.)Dunal]品种‘Mango’为实验材料,从100个ISSR(Inter-simple sequence repeat)引物中筛选出6个多态性高、重复性好的引物。利用这6条引物,对13个泡泡树品种及其半同胞家系和6个种源的48个样品进行遗传多样性分析。通过DNA检测,扩增出2 337条谱带,其中多态性条带为1 930,多态性比率为82.6%。聚类分析结果表明,供试材料相似系数介于0.59~0.99之间。供试材料在相似系数0.91处的DNA水平上的类群划分和形态特征一致。该研究为泡泡树新品种培育的亲本选择及杂种鉴定提供理论依据。
译  名:
Development of ISSR Markers and Analysis of Genetic Diversity of Asimina triloba
作  者:
YOU Xiangliang;LUO Yulan;YIN Lijuan;
单  位:
YOU Xiangliang%LUO Yulan%YIN Lijuan
关键词:
Asimina triloba;;variety;;ISSR;;genetic diversity;;clustering analysis
摘  要:
Asimina triloba(L.) Dunal ‘Mango' was used as the experimental material,48 A.trilobasamples were analyzed by ISSR(Inter-simple sequence repeat)technique to study their taxonomic and phylogenetic relationships.6 polymorphic and reproducible primers were screened from 100 primers.A total of 2 337 ISSR bands were amplified,of which 1 930 were polymorphic bands,and the polymorphic rate was 82.6%.According to ISSR-PCR results,a cluster analysis(UPGMA)was conducted to generate a dendrogram.The results showed that the similarity coefficient of the tested materials were between 0.59~0.99.The test materials were divided into 19 groups at the level of 0.91.The genetic diversity and morphological characteristics of Asimina triloba and its varieties at the DNA level were more consistent.This study provided a theoretical basis for parents selection and hybrid identification of new bubble trees breeding in Asimina triloba.

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