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Position: Home > Articles > Progress in ecdysone receptor (EcR) and insecticidal mechanisms of ecdysteroids Acta Entomologica Sinica 2007,50 (1) 70-76

昆虫蜕皮激素受体及其类似物的杀虫机制研究进展

作  者：
刘永杰;徐蓬军;李艳伟;束怀瑞;黄大卫
单  位：
山东农业大学园艺学院;山东农业大学植物保护学院昆虫分子生物学实验室
关键词：
蜕皮激素受体;超气门蛋白;配体结合域;级联反应;活性筛选;基因开关
摘  要：
昆虫的蜕皮、变态和繁殖受到蜕皮激素的严格调控。蜕皮激素作用靶标由蜕皮激素受体(ecdysteroidreceptor,EcR)和超气门蛋白(ultraspiracleprotein,USP)组成,蜕皮激素与EcR/USP作用启动蜕皮级联反应过程。昆虫EcR具有种类或类群的特异性,研究其结构、功能和调控机理在开发环境友好型新药剂和基因调控开关等方面具有重要指导作用。该文介绍了昆虫EcR的结构和功能特点,蜕皮激素及其类似物与EcR/USP的分子作用方式,以及基于EcR/USP的新杀虫剂创制和基因调控开关设计等方面的重要进展。
译  名：
Progress in ecdysone receptor (EcR) and insecticidal mechanisms of ecdysteroids
作  者：
LIU Yong-Jie1, XU Peng-Jun1, LI Yan-Wei1, SHU Huai-Rui2, HUANG Da-Wei 1, 3,*(1. Laboratory of Insect Molecular Biology, Shandong Agricultural University, Tai'an, Shandong 271018, China; 2. College of Horticulture, Shandong Agricultural University, Tai'an, Shandong 271018, China; 3. Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China)
关键词：
Ecdysteroid receptor (EcR); ultraspiracle protein (USP); ligand binding domain; genetic cascade; screening assays; gene switch
摘  要：
Ecdysteroids, the steroid hormones, play a crucial role in coordinating molting, metamorphosis and reproduction in insects. The action of ecdysteroids, mainly 20-hydroxyecdysone (20E), is mediated by their binding to the ecdysone receptor (EcR), which requires a heterodimeric partner, ultraspiracle protein (USP). Upon binding the ligand, the EcR-USP heterodimer binds to a DNA ecdysone response element (EcRE) that is located in the promoter region of a series of ecdysteroid-responsive genes and thereafter triggers the expression of a cascade of genes involved in regulating some key developmental events in insects. The EcR has been an important target in designing new, environmentally safe insecticides against pest species. The differences in the ligand binding domains (LBD) of EcRs from insects belonging to different orders can be exploited to discover order-specific insecticides. The EcR also has been used to develop inducible gene switches for efficient regulation of foreign genes in applications such as gene therapy, protein production and functional genomics. The structural and function characteristics of EcRs, the mode of molecular action of ecdysteroid (PonA) and diacylhydrazine (BYI06830) with EcR/USP respectively, and EcR-based screening assays and gene switches were reviewed in this article.

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