中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 家蚕蜕皮激素受体基因EcR-B1的启动子调控区域活性检测 蚕业科学 2014 (3) 33-39
Position: Home > Articles > Activity Detection on Promoter Regulatory Region of Ecdysone Receptor Gene EcR-B1 in the Silkworm,Bombyx mori Science of Sericulture 2014 (3) 33-39

家蚕蜕皮激素受体基因EcR-B1的启动子调控区域活性检测

作  者：
黄明霞;杜杰;刘云垒;赵国栋;卫正国
单  位：
苏州大学蚕桑研究所;苏州大学基础医学与生物科学学院现代丝绸国家工程实验室;苏州大学
关键词：
家蚕;蜕皮激素受体;启动子;双荧光素酶报告系统;转录调控
摘  要：
昆虫蜕皮激素通过与蜕皮激素受体(EcR)和超气门蛋白(USP)形成的异源二聚体相互作用,起始包括转录因子表达在内的蜕皮级联反应,引起昆虫蜕皮。为了探究家蚕蜕皮激素受体基因EcR-B1(BmEcR-B1)表达的调控机制,利用双荧光素酶报告系统和Bac-to-Bac表达系统检测家蚕幼虫经蜕皮激素诱导后,脂肪体、马氏管、中肠组织内不同缺失片段的BmEcR-B1启动子活性。对各组织样品的检测结果均证明:BmEcR-B1启动子-138~-11 bp之间存在正调控元件,并且其活性能被蜕皮激素诱导。在细胞水平上进一步分析BmEcR-B1启动子调控区域,结果显示在-138~-108 bp区域包含的调控元件对于BmEcR-B1启动子的转录调控是必需的,序列分析显示这一区域包含有畸形基因编码蛋白Dfd和热休克转录因子(HSF)2个转录调控元件的结合位点。研究结果为探索BmEcR-B1在家蚕变态发育中的转录调控机制提供了有益线索。
译  名：
Activity Detection on Promoter Regulatory Region of Ecdysone Receptor Gene EcR-B1 in the Silkworm,Bombyx mori
作  者：
HUANG Ming-Xia;DU Jie;LIU Yun-Lei;ZHAO Guo-Dong;WEI Zheng-Guo;SHEN Wei-De;School of Basic Medicine and Biological Sciences,Soochow University;National Engineering Laboratory for Modern Silk;
关键词：
Bombyx mori;;Ecdysone receptor;;Promoter;;Dual-luciferase assay;;Transcriptional regulation
摘  要：
Ecdysteroid interacts with the heterodimer formed between ecdysone receptor(EcR) and ultraspiracle protein(USP) to initiate the cascade reactions including the expression of transcription factors that result in insect molting. To analyze the regulatory mechanism of EcR-B1 gene expression in silkworm(Bombyx mori),promoter activity of EcR-B1 gene with various deletions in the promoter regulatory region was determined using the dual luciferase reporter assay and Bac-to-Bac expression system in fat body,Malpighian tubule and midgut of silkworm larvae after induction with ecdysone(20E). The results showed that positive regulatory elements existed at position-138 to-11 bp in BmEcR-B1 gene region,of which activity could be induced by ecdysone. Further analysis of the BmEcR-B1 promoter at cellular level suggested that region-138 to-108 bp contained important regulatory elements that were necessary for transcriptional regulation of BmEcR-B1 promoter. Sequence analysis revealed that these regions had several transcriptional regulatory elements including Drosophila Dfd and HSF transcription factor binding sites. This study provides a useful clue for investigating transcriptional regulation mechanism of BmEcR-B1 gene during metamorphosis of silkworm.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46