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当前位置: 首页 > 文章 > 基于条形码ITS2序列的青天葵及其混伪品分子鉴别 生物技术通报 2012,0 (12) 173-179
Position: Home > Articles > Molecular Identification of Nervilia fordii(Hance) Schltr.and Its Adulterants Based on 1TS2 DNA Barcodes Biotechnology Bulletin 2012,0 (12) 173-179

基于条形码ITS2序列的青天葵及其混伪品分子鉴别

作  者：
黄琼林;马新业;何瑞;詹若挺;陈蔚文
单  位：
广州中医药大学中药资源科学与工程研究中心
关键词：
青天葵;ITS2混伪品;分子鉴别
摘  要：
通过分析青天葵及其常见混伪品的ITS2序列,建立青天葵新型真伪鉴别方法。采用植物基因组DNA提取试剂盒提取青天葵及其混伪品的叶片DNA,一对通用引物PCR扩增ITS2基因片段并直接双向测序。采用DNAMAN、ClustalX软件拼接比对序列,MEGA4.0软件构建NJ树,Schultz等建立的数据库和网站预测lTS2序列的二级结构。结果显示,获得的12条ITS2序列的长度范围为203-242 bp,GC含量范围为53.1%-71.8%。所有样品ITS2序列比对后的长度为249 bp,其中存在226个变异位点。青天葵种间K2P遗传距离(1.125)远大于种内K2P遗传距离(0.004)。基于ITS2的序列的NJ树和二级结构均能直观地区分青天葵及其混伪品。
译  名：
Molecular Identification of Nervilia fordii(Hance) Schltr.and Its Adulterants Based on 1TS2 DNA Barcodes
作  者：
Huang Qionglin Ma Xinye He Rui Zhan Ruoting Chen Weiwen (Research Center of Chinese Herbal Resource Science and Engeering,Key Laboratory of Chinese Medicinal Resource from Lingnan,Guangzhou University of Chinese Medicine,Ministry of Education,Guangzhou 510006)
关键词：
Nervilia fordii(Hance ) Schltr.;;ITS2 adulterant;;Molecular identification
摘  要：
It was to establish a novel identification method for Nervilia fordii and its adulterants through ITS2 sequence analysis.Total DNA was extracted from fresh leaves,and ITS2 region was amplified and sequenced with a pair of universal primers.The sequences were assembled and aligned by DNAMAN and CLUSTALX,and NJ tree was constracted using MEGA 4.0.Secondary structures of ITS2 sequences were predicated in the database built by Schultz et al.Significant differences were observed in the ITS2 sequences between Nervilia fordii and its adulterants.The interspecific genetic distance was far more than the intraspecific disatance.Both NJ tree and secondary structure based on ITS2 sequences can distinguish Nervilia fordii and its adulterants intuitively.ITS2 is an effective biomarker to identify Nervilia fordii and its adulterants.

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