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Position: Home > Articles > Separation and in vitro culture of mouse spermatogenic cells Animal Husbandry & Veterinary Medicine 2017,49 (2) 15-17

小鼠生精细胞的分离和体外培养

作  者:
王香祖;席继锋;贾斌
单  位:
石河子大学动物科技学院;新疆农业职业技术学院
关键词:
小鼠;生精细胞;分离;体外培养
摘  要:
为了建立一种操作性强、稳定、高效的小鼠生精细胞体外培养体系,从10只8周龄成年雄性健康清洁级昆明小鼠睾丸组织分离出细胞得到混悬液,采用支持细胞和生精细胞共培养方式,通过添加多种细胞培养液,以维持细胞的生长和增殖。结果表明:采用睾丸生精细胞、支持细胞共同培养的方式可得到密度较大的生精细胞,且精原细胞、精母细胞的数量占绝大多数。
译  名:
Separation and in vitro culture of mouse spermatogenic cells
作  者:
WANG Xiangzu;XI Jifeng;JIA Bin;Xinjiang Agricultural Vocational Technical College;College of Animal Science and Technology,Shihezi University;
关键词:
mouse;;spermatogenic cell;;separation;;in vitro culture
摘  要:
The present study was conducted to develop a simple,stable and efficient in vitro culture system for mouse germ cells.Testiculus organization originally from 10 Kunming mice at 8 weeks were got and adopt to suspension liquid,and co-cultured with Sertoli cells and germ cells together and adding the various cell culture solutions keeping cell growth and survive.The results showed that the co-culturing system of Sertoli cells and germ cells were preferable.

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