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当前位置: 首页 > 文章 > 不同激素对四种草地早熟禾种子愈伤组织的诱导 北方园艺 2018 (21) 66-71
Position: Home > Articles > Induction of Seed Callus From Four Kinds of Kentucky Bluegrass Seeds With Different Hormones Northern Horticulture 2018 (21) 66-71

不同激素对四种草地早熟禾种子愈伤组织的诱导

作  者：
陈阳;孙华山;金一锋;王佳贺;陈雅君
关键词：
草坪草;草地早熟禾;组织培养;愈伤组织
摘  要：
以4种草地早熟禾(Poa pratensis L.)为试材,以成熟种子为外植体,筛选了适宜的消毒方法及各品种适宜的愈伤组织诱导培养方式,为后续建立高效便捷的草地早熟禾再生体系提供依据。结果表明:最佳的消毒方式为1%NaClO消毒7min,蒸馏水清洗4～5遍,再用无菌水浸种12h,接种前用75%乙醇消毒40s,用无菌水清洗4～5遍,1%NaClO消毒10min后接种;4种草地早熟禾基因型不同,其诱导愈伤组织的出愈率有显著差异(P<0.05);"午夜2号"早熟禾种子诱导最适宜培养基为MS+1mg·L-1 2,4-D+0.1mg·L-16-BA,"公园"与"黑虎"早熟禾种子诱导最适宜培养基为MS+6mg·L-1 2,4-D,"雪狼"早熟禾种子诱导最适宜培养基为MS+1mg·L-1 2,4-D。
译  名：
Induction of Seed Callus From Four Kinds of Kentucky Bluegrass Seeds With Different Hormones
作  者：
CHEN Yang;SUN Huashan;JIN Yifeng;WANG Jiahe;CHEN Yajun;College of Horticulture and Garden,Northeast Agricultural University;College of Life Science and Agriculture,Qiqihar University;
单  位：
CHEN Yang%SUN Huashan%JIN Yifeng%WANG Jiahe%CHEN Yajun%College of Horticulture and Garden,Northeast Agricultural University%College of Life Science and Agriculture,Qiqihar University
关键词：
turf grass;;Kentucky bluegrass;;tissue culture;;callus
摘  要：
In this study,four Kentucky bluegrass varieties were used as materials for the screening of appropriate sterilization and callus induction methods with the mature seeds as explants,in order to supply reference for bluegrass use.The results showed that,the optimal sterilization method was to treat the seeds by 1% NaClO for 7 minutes followed by 4-5 times of washing by distilled water.Subsequently,the seeds were immersed with sterile water for 12 hours.Right before inoculation,the seeds were again washed with 75%ethanol for 40 seconds and then with sterile water for 4-5 times,followed by 10 minutes sterilization by 1% NaClO.The four Kentucky bluegrass varieties possessed different genotypes as well as significantly different frequencies of callus induction(P<0.05).The optimal culture medium was MS+1 mg·L-1 2,4-D+0.1 mg·L-1 6-BA for Kentucky bluegrass‘Midnight II',MS+6 mg·L-1 2,4-D for Kentucky bluegrass‘Park'and‘Black Tiger',and MS+1 mg·L-1 2,4-D for Kentucky bluegrass‘Snow Wolf',respectively.

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