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玉蝉花愈伤组织的诱导及植株再生研究

作  者:
王玲;唐彪;曹柏;宋红
关键词:
玉蝉花;茎尖;愈伤组织;再分化;不定芽
摘  要:
以玉蝉花的茎尖和嫩叶为外植体进行愈伤组织诱导和再分化研究,成功建立了通过茎尖愈伤诱导途径的植株再生体系.研究得出:玉蝉花茎尖诱导愈伤的最佳培养基为MS+6-BA 1.0mg/L+NAA 0.5mg/L+2,4-D 1.0mg/L,最高诱导率为51.51%;不定芽诱导的培养基为MS+6-BA 1.0mg/L+NAA 0.5mg/L+KT 1.0mg/L,分化率达到72.31%,平均分化不定芽数为5.81;不定芽生根最佳培养基为MS+NAA 1.5mg/L,生根率为100%.
作  者:
WANG Ling;TANG Biao;CAO Bai;SONG Hong;The College of Landscape,Northeast Forestry University;
单  位:
WANG Ling%TANG Biao%CAO Bai%SONG Hong%The College of Landscape,Northeast Forestry University
关键词:
Iris ensata;;shoot tip;;callus;;differentiation;;adventitious shoot
摘  要:
Take shoot tip and leaf base of Iris ensata as explants for callus induction and differentiation research.We successfully established plantlet regeneration system through the method of induction of shoot tips.The study concluded that the best medium for callus induction by shoot tip of Iris ensata was MS+6-BA1.0 mg/L+NAA0.5 mg/L+2,4-D 1.0 mg/L,the highest induction rate was 51.51%;The medium for induction of adventitious shoot was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+KT 1.0 mg/L,the differentiation rate was 72.31%,The average number of adventitious shoot was 5.81;The best medium for rooting of adventitious shoot was MS+NAA 1.5 mg/L and the rooting rate was100%.

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