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Position: Home > Articles > Isolation,Culture and Identification of Endothelial Progenitor Cells Biotechnology Bulletin 2010,0 (2) 24-27+32

内皮祖细胞的分离培养与鉴定

作  者:
白春雨;侯玲玲;庞全海;关伟军;马月辉
单  位:
山西农业大学动物科技学院;中国农业科学院北京畜牧兽医研究所;北京交通大学生命科学与生物工程研究院
关键词:
内皮祖细胞;分离;鉴定
摘  要:
内皮祖细胞的分离方法有免疫磁珠分离法、淋巴细胞分离液分离法(1.077)和差速贴壁法,这3种方法已被人们广泛使用,均可分离到一定的目的细胞。分离到的目的细胞在培养过程中逐渐分化、成熟、发育为内皮细胞。在内皮细胞和内皮祖细胞的鉴别区分,使用CD34+/CD133+/KDR+鉴定为内皮祖细胞,同时使用内皮祖细胞吞噬D il-ac-LDLFITC-UEA双阳性的方法也可鉴定为内皮祖细胞。
译  名:
Isolation,Culture and Identification of Endothelial Progenitor Cells
作  者:
Bai Chunyu 1,2 Hou Lingling3 Pang Quanhai2 Guan Weijun1 Ma Yuehui1(1 Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100193;2 College of Animal Science and Veterinary Medicine,Shanxi Agriculture University,Taigu 030801;3 Institute of Life Science and Bioengineering,Beijing Jiaotong University,Beijing 100044)
关键词:
Endothelial progenitor cells Isolation Identification
摘  要:
There are three isolation methods of endothelial progenitor cells,including immunomagnetic beads,lymphocyte separating medium(1.077) and differential adhesion,which has been widely used and could isolate a certain number of the desired cells.These cells gradually differentiate,mature,and develope into endothelial cells in culture process.In identification,cells with CD34+/CD133+/KDR+ are identified as endothelial progenitor cells,and at the same time,the double positive phagocytosis assay of Dil-ac-LDLFITC-UEA also help to identify endothelial progenitor cells.

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