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当前位置: 首页 > 文章 > 柞蚕蛹虫草抑制人肝癌SMMC-7721细胞增殖和诱导凋亡的作用 蚕业科学 2011,37 (1) 160-164
Position: Home > Articles > Cordyceps Militaris of Antheraea pernyi Inhibits Proliferation and Promotes Apoptosis of Hepatoma SMMC-7721 Cells Science of Sericulture 2011,37 (1) 160-164

柞蚕蛹虫草抑制人肝癌SMMC-7721细胞增殖和诱导凋亡的作用

作  者：
王林美;都兴范;李学军;米锐;李亚洁;李树英
单  位：
辽宁省农业科学院大连生物技术研究所
关键词：
柞蚕蛹虫草;人肝癌细胞;凋亡;细胞周期
摘  要：
为鉴定柞蚕蛹虫草新的医用价值,将不同浓度的柞蚕蛹虫草水提物(AEoAPC)分别作用于体外培养的人肝癌SMMC-7721细胞,于24、48、72 h后采用倒置相差显微镜和透射电子显微镜观察、四甲基偶唑蓝(MTT)比色及流式细胞术(FCM)检测细胞的增殖和凋亡情况。结果表明:细胞培养板中分别加入质量浓度为0.1、0.2、0.5、0.8、1.0、2.0、5.0 g/L的AEoAPC,均能显著抑制SMMC-7721细胞的增殖,并呈现时间剂量依赖性,1.0 g/L AEoAPC处理24、48、72 h后,对SMMC-7721细胞的生长抑制率分别为18.9%、46.4%、77.2%;经AEoAPC处理后大量癌细胞出现染色质边集和凝聚、核碎裂及凋亡小体;1.0 g/LAEoAPC处理24、48、72 h SMMC-7721细胞凋亡率分别为7.65%、11.04%、23.02%,细胞凋亡程度与AEoAPC作用时间呈正相关,有丝分裂第一间距期(G1)的细胞在AEoAPC作用72 h后分布明显增加。研究结果初步显示,AEoAPC对人肝癌SMMC-7721细胞有抑制增殖和诱导凋亡的作用,其抗肿瘤机制与诱导肿瘤细胞凋亡有关。
译  名：
Cordyceps Militaris of Antheraea pernyi Inhibits Proliferation and Promotes Apoptosis of Hepatoma SMMC-7721 Cells
作  者：
WANG Lin-Mei DU Xing-Fan LI Xue-Jun MI Rui LI Ya-Jie LI Shu-Ying(Dalian Institute of Biotechnology,Liaoning Academy of Agricultural Sciences,Dalian Liaoning 116024,China)
关键词：
Cordyceps militaris;Human hepatoma cells;Apoptosis;Cell cycle
摘  要：
To evaluate new medical value of the cordyceps militaris of Antheraea pernyi,the human hepatoma SMMC-7721 cells were treated with different concentrations of the aqueous extract of A.pernyi cordycep militaris(AEoAPC) for 24,48,and 72 h respectively in vitro.The proliferation and apoptosis of the hepatoma SMMC-7721 cells were examined by an inverted phase contrast microscope,a transmission electron microscope,methyl thiazolyl tetrazolium(MTT) colorimetric assay,and flow cytometry(FCM).The results showed that addition of AEoAPC into cell culture plates with mass concentrations of 0.1,0.2,0.5,0.8,1.0,2.0,and 5.0 g/L respectively could inhibit proliferation of SMMC-7721 cells significantly and the inhibition was in a time and dose-dependent manner.Treatment with 1.0 g/L AEoAPC for 24,48,and 72 h had an inhibitory rate to hepatoma SMMC-7721 cell growth of 18.9%,46.4%,and 77.2% respectively.After AEoAPC treatment,a large number of cancer cells showed chromatin margination,chromatin condensation,nuclear fragmentation and apoptotic bodies.SMMC-7721 cell apoptotic rate from treatment with 1.0 g/L AEoAPC for 24,48,and 72 h was 7.65%,11.04%,and 23.02% respectively.The degree of apoptosis had positive correlation with AEoAPC treatment duration,and the cell cycle of SMMC-7721 was apparently arrested at G1 phase by AEoAPC treatment for 72 h.Our results suggested that AEoAPC could inhibit proliferation and induce apoptosis of the hepatoma SMMC-7721 cells,and its antitumor function was related with induction of apoptosis to tumor cells.

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