中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from 'Candidatus Liberibacter asiaticus' 农业科学学报 (英文) 2015,14 (10)
Position: Home > Articles > High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from 'Candidatus Liberibacter asiaticus' Journal of Integrative Agriculture 2015,14 (10)

High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from 'Candidatus Liberibacter asiaticus'

作  者：
Wang Xue-feng;Chen Jiao-yue;Tan Jin;Duan Suo;Deng Xiao-ling;Chen Jian-chi;Zhou Chang-yong
单  位：
Southwest Univ, Coll Plant Protect, Chongqing 400715, Peoples R China;Southwest Univ, Chinese Acad Agr Sci, Natl Citrus Engn Res Ctr, Citrus Res Inst, Chongqing 400712, Peoples R China;South China Agr Univ, Citrus Huanglongbing Res Ctr, Guangzhou 510642, Guangdong, Peoples R China;USDA ARS, SJVASC, Parlier, CA 93648 USA
关键词：
Huanglongbing;'Candidatus Liberibacter asiaticus';miniature inverted-repeat transposable element
摘  要：
Two miniature inverted-repeat transposable elements (MITEs), MCLas-A and MCLas-B, were recently identified from 'Candidatus Liberibacter asiaticus' known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). MCLas-A was suggested as an active MITE because of its mobility. The immediate upstream gene of the two MITEs was predicted to be a putative transposase. The goal of this study is to analyze the sequence variation in the upstream putative transposase of MITEs and explore the possible correlation between sequence variation of transposase gene and MITE activity. PCR and sequence analysis showed that 12 sequence types were found in six major amplicon types from 43 representative 'Ca. L. asiaticus' isolates from China, the United States and Brazil. Out of the 12 sequence types, three (14, T5-2, T6) were reported for the first time. Recombination events were found in the two unique sequence types (T5-2 and 16) which were detected in all Brazilian isolates. Notably, no sequence variation or recombination events were detected in the upstream putative transposase gene of MCLas-A, suggesting the conservation of the transposase gene might be closely related with the MITE activity. Phylogenetic analysis demonstrated two well supported clades including five subclades were identified, clearly reflecting the geographical origins of isolates, especially that of Ruili isolates, Sao Paulo isolates and a few Florida isolates.

相似文章

导出引用
计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46