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Position: Home > Articles > Decreasing detection frequency of MITE (MCLas-A) in the population of 'Candidatus Liberibacter asiaticus' recently collected in southern China Journal of Integrative Agriculture 2020,19 (10)

Decreasing detection frequency of MITE (MCLas-A) in the population of 'Candidatus Liberibacter asiaticus' recently collected in southern China

作  者:
Cui Xue-jin;Zeng Chun-hua;Liu Ke-hong;Teng Cal-ling;Zhou Chang-yong;Wang Xue-feng
单  位:
Southwest Univ, Natl Citrus Engn Res Ctr, Citrus Res Inst, Chinese Acad Agr Sci, Chongqing 400712, Peoples R China
关键词:
Huanglongbing;'Candidatus Liberibacter asiaticus';miniature inverted-repeat transposable element;MITE
摘  要:
An active miniature inverted-repeat transposable element (MITE), MCLas-A, was previously identified from 'Candidatus Liberibacter asiaticus' known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). To explore the recent transposition status of MCLas-A, 389 'Ca. L. asiaticus' strains collected from nine regions in China were amplified using a specific primer set and three representative 'Ca. L. asiaticus' strains were analyzed by next-generation sequencing (NGS) approach. PCR and genomic analysis showed that the entire MCLas-Awas only present in 1.80% (7/389) and the jumping-out type of the MITE was predominant (81.23%) in samples tested, suggesting high frequency transposition occurred in 'Ca. L. asiaticus' strains recently collected from China. Biological roles of transposition of the active MITE remain to be determined.

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