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当前位置: 首页 > 文章 > Oct4,Sox2,Nanog和c-Myc基因在蚯蚓发育早期的表达 热带生物学报 2015,6 (2) 134-140
Position: Home > Articles > Expression of Genes Oct4,Sox2,Nanog and c-Myc of the Earthworm Eisenia fetida at the Early Development Stage Journal of Tropical Biology 2015,6 (2) 134-140

Oct4,Sox2,Nanog和c-Myc基因在蚯蚓发育早期的表达

作  者：
郑鹏飞;刁晓平;宋芹芹;杜俊俏;程华民;王海花;张禹
单  位：
海南大学农学院;海南大学环境与植物保护学院
关键词：
赤子爱胜蚓;Sox2;克隆;发育;Oct4
摘  要：
通过已知物种的干细胞多能性基因核酸序列,利用同源序列法,成功克隆得到赤子爱胜蚓(Eisenia foetida)的Oct4,Nanog,Sox2和c-Myc 4个基因,并通过实时荧光定量PCR分析了上述4个基因在赤子爱胜蚓茧孵化期间的表达情况。结果表明:赤子爱胜蚓茧在孵化期0~21 d,4个基因呈相似的规律,表达量均逐步升高,在21 d达到峰值;在幼虫期Oct4,Nanog和c-Myc 3个基因的表达量高于孵化期。结果表明,上述基因在赤子爱胜蚓早期发育过程中表达模式具有一定的相似性。
译  名：
Expression of Genes Oct4,Sox2,Nanog and c-Myc of the Earthworm Eisenia fetida at the Early Development Stage
作  者：
ZHENG Pengfei;DIAO Xiaoping;SONG Qinqin;DU Junqiao;CHENG Huamin;WANG Haihua;ZHANG Yu;College of Agronomy,Hainan University;Haikou Key Laboratory of Environment Toxicology;College of Environment and Plant Protection,Hainan University;
关键词：
Eisenia foetida;;Sox2;;clone;;development;;Oct4
摘  要：
Stem cell pluripotency genes play a key role in the early development of animals. According to known species' stem cell pluripotency genes,four genes( Oct4,Nanog,Sox2 and c-Myc) were cloned by using the homologue-sequence method from the earthworm Eisenia foetida. The expression of these four genes was detected by the real-time fluorescence quantitative PCR in the earthworm E. foetida at the early development stage. The expression of the four genes was escalated gradually from day 0 to 21,the highest at 21 d at the cocoon hatching stage,but declined at day 28. Three genes Oct4,Nanog and c-Myc were detected to have higher expression at the larval stage than at the cocoon hatching stage. This suggested that the four genes had similar expression pattern and might have a synergistic effect during the early development of Eisenia fetida.

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