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当前位置: 首页 > 文章 > La对UV-B辐射下大豆幼苗NRA和可溶性蛋白的影响 中国油料作物学报 2006,28 (3) 298-301
Position: Home > Articles > Effect of La~(3+) on soybean seedling nitrate reductase activity and soluble protein content under supplemental UV-B radiation Chinese Journal of Oil Crop Sciences 2006,28 (3) 298-301

La对UV-B辐射下大豆幼苗NRA和可溶性蛋白的影响

作  者：
薛隽;刘文;张光生;周青
单  位：
江南大学工业生物技术教育部重点实验室
关键词：
氯化镧;紫外辐射;大豆幼苗;硝酸还原酶;可溶性蛋白
摘  要：
在温室模拟增强紫外辐射UV-B(280~320nm)环境下,研究镧(La)对UV-B胁迫下大豆幼苗叶片硝酸还原酶活性(N itrate reductase activity,NRA)、可溶性蛋白、淀粉和可溶性糖含量的影响。结果显示,高低两个紫外强度剂量下,叶片NRA较对照组都有显著下降,处理5d后降幅分别达到40.9%和42.0%;而经过适宜浓度LaC l3(20mg/L)预处理后,降幅较UV-B单独处理组均有明显升高,分别为39.2%和38.9%。蛋白质、淀粉和可溶性糖含量变化与NRA相似,但是紫外辐射处理组蛋白含量变化较对照组呈现先升高后下降趋势。结果表明适宜浓度的La3+能够促进NRA以及UV-B胁迫下NRA活性的提高,缓解UV-B胁迫对植物的伤害。
译  名：
Effect of La~(3+) on soybean seedling nitrate reductase activity and soluble protein content under supplemental UV-B radiation
作  者：
XUE Juan,LIU Wen,ZHANG Guang-sheng,ZHOU Qing(The Key Laboratory of Industrial Biotechnology,Ministry of Education,Southern Yangtze university,Wuxi 214036,China)
关键词：
Lanthanum Chloride;Ultraviolet-B radiation;Soybean seedling;Nitrate reductase activity;Soluble protein
摘  要：
Effect of La~(3+) on the leaf nitrate reductase activity(NRA) and the contents of soluble protein,soluble sugar and starch in soybean seedlings were studied under elevated ultraviolet-B radiation(UV-B,280~320nm).The result showed that NRA decreased significantly under the two different doses of UV-B treatment,with a decrease of 40.9% and 42.0% compared to the control after 5 days of treatment.But after pretreated with 20mg/L LaCl_3,the NRA increased by 5%~10% compared to that treated by UV-B radiation only.The same changes were found with contents of starch and soluble sugar as NRA.But protein content only had a little difference,which increased after UV-B treatment in the beginning 3 days and decreased in following 2 days.Our results indicated that 20mg/L LaCl_3 had an effect to increase leaf NRA treated by UV-B,and alleviated seedling damage caused by UV-B.

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