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Position: Home > Articles > Effect of Leucine on Proliferation and Cell Cycle of Porcine Trophectoderm Cell Chinese Journal of Animal Science 2016,52 (7) 35-40

亮氨酸对猪胎盘滋养层细胞增殖和周期的影响

作  者:
刘炀;王旭贞;管武太;王超先;陈芳
单  位:
山西省畜牧兽医学校;华南农业大学动物科学学院
关键词:
亮氨酸;胎盘滋养层细胞;细胞增殖;细胞周期
摘  要:
为了研究亮氨酸(Leu)对猪胎盘滋养层细胞(p Tr)增殖数目和增殖周期的影响,本试验分别利用MTT法和流式细胞仪检测不同Leu浓度(0、0.01、0.05、0.1、0.25、0.5、1、5、10 mmol/L)处理p Tr不同时间(24 h、48 h、72 h)后,其增殖活力和细胞周期分布。结果显示,Leu处理p Tr细胞24 h后,0.01 mmol/L组和0.05 mmol/L组细胞数目极显著降低(P<0.01);Leu处理p Tr细胞48 h后,细胞数目极显著降低(P<0.01);1~10 mmol/L Leu处理p Tr细胞72 h后,细胞数目极显著降低(P<0.01)。不同浓度Leu处理p Tr 24 h和48 h后,各试验组G0~G1期细胞数目逐渐增多,S期细胞数目逐渐减少,并有时间和剂量依赖性。高浓度的Leu会阻碍正常细胞周期,从而抑制p Tr的增殖活力。
译  名:
Effect of Leucine on Proliferation and Cell Cycle of Porcine Trophectoderm Cell
作  者:
LIU Yang;WNAG Xu-zhen;GUAN Wu-tai;WANG Chao-xian;CHEN Fang;College of Animal Science, South China Agricultural University;School of Shanxi Animal Science and Veterinary Medicine;
关键词:
leucine;;porcine trophectoderm cell;;proliferation;;cell cycle
摘  要:
To investigate the effect of leucine on proliferation and cell cycle of porcine trophectoderm cell(p Tr), MTT and flow cytometer were used to detect the proliferation and cell cycle of p Tr after treatment with different leucine concentrations(0, 0.01, 0.05, 0.1, 0.25, 0.05, 1, 5, 10 mmol/L) and time(24 h, 48 h, 72 h). The results showed that,after treatment with Leu for 24 h, the cell number of 0.01mmol/L and 0.05mmol/L groups decreased very significantly(P<0.01); the cell number decreased very significantly when treated with Leu for 48h( P<0.01). After treatment with 1-10 mmol/L Leu for 72 h, the cell number decreased very significantly. After treatment with different concentrations for24 h and 48 h, compaired with the control group, the cell number at phase G0-G1 incresed and at phase S decreased in a dose- and time-dependent manner. In conclusion, high concentratin of leucine can suppress the proliferation of p Trby blocking cell cycle progression.

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