中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 烟叶样品中18种氨基酸的微波水解分析方法研究 西南农业学报 2014,27 (6) 2629-2634
Position: Home > Articles > Study on Analysis Method for 18 Amino Acids of Tobacco Samples Southwest China Journal of Agricultural Sciences 2014,27 (6) 2629-2634

烟叶样品中18种氨基酸的微波水解分析方法研究

作  者：
周虹;罗玲;郭灵安;毛建霏;黄世群;顾会战;陈金相
单  位：
四川省农业科学院分析测试中心;四川省广元市烟叶生产技术推广应用中心
关键词：
微波水解;烟叶样品;氨基酸
摘  要：
氨基酸在烟叶生长过程的不同阶段会发生不同的变化,影响烟叶品质。为控制烟叶质量,快速、准确测定烟叶氨基酸,本文采用了微波水解烟叶样品,氨基酸自动分析仪钠离子系统测定18种氨基酸。通过微波水解的条件试验确定了微波水解烟叶最佳条件:烟叶称样量30~60 mg,水解温度160℃,保温时间20 min,该方法测定18种氨基酸回收率在70.1%~109.4%,与常规酸水解方法回收率相当,准确度高。相对标准偏差0.83%~10.10%与常规酸水解法接近,该方法快捷方便、节能、准确,适合烟叶水解氨基酸的测定。
译  名：
Study on Analysis Method for 18 Amino Acids of Tobacco Samples
作  者：
ZHOU Hong;LUO Ling;GUO Ling-an;MAO Jian-fei;HUANG Shi-qun;GU Hui-zhan;CHEN Jin-xiang;Analysis and Testing Center of Sichuan Academy of Agricultural Science;Laboratory of Quality & Safety Risk Assessment for Agro-products (Chengdu),Ministry of Agriculture;Analysis and Testing Center of Sichuan Academy of Agricultural Science;Laboratory of Quality & Safety Risk Assessment for Agro-products (Chengdu),Ministry of Agriculture;Promotion and Application Center for Tobacco Production Technology of Guangyuan;
关键词：
Microwave hydrolysis;;Tobacco sample;;Amino acids
摘  要：
Amino acid contents,which affect the quality of tobacco,vary during the different stages of tobacco growth process. In order to quantify the amino acids fast and accuratly for quality control of tobacco,herein,18 amino acids of tobacco samples were analyzed with sodium ion system of automatic amino acid analyzer and microwave hydrolysis. The optimum condition for microwave hydrolysis of tobacco amino acids was obtained from condition tests: sample weight 30- 60 mg,hydrolysis temperature 160 ℃,holding time 20 min. The average recoveries and relative standard deviations were 70. 1 %- 109. 4 %,and 0. 83 %- 10. 1 %,respectively,which were comparable with conventional acid hydrolysis method. The proposed method is facial,accurate,energy saving and suitable for the hydrolytic amino acids determination of tobacco.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46