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影响分光光度法测定钝顶螺旋藻干粉叶绿素含量的因素分析

作  者:
罗光宏;许耀照;何琳
单  位:
张掖市科学技术局;甘肃省微藻工程技术研究中心;河西学院河西生态与绿洲农业研究院
关键词:
钝顶螺旋藻;分光光度法;干粉;叶绿素含量
摘  要:
为快速检测藻类干粉叶绿素含量,以钝顶螺旋藻干粉为材料,在不同浸提剂、不同浸提时间和不同浸提温度处理条件下采用分光光度法测定其叶绿素、叶绿素a和类胡萝卜素含量。结果表明:钝顶螺旋藻干粉不同浸提剂处理条件下叶绿素含量依次为95%乙醇-99.5%丙酮(1∶1,V/V)>95%乙醇-99.5%丙酮(2∶1,V/V)>95%乙醇-99.5%丙酮(1∶2,V/V)>99.5%丙酮>95%乙醇;不同浸提时间条件下测定叶绿素含量依次为8 h>6 h>4 h>14 h>12 h>10 h>24 h>2 h;不同浸提温度条件下测定叶绿素含量依次为21℃>22℃>20℃>15℃>25℃。分光光度法测定钝顶螺旋藻干粉叶绿素含量最优条件是浸提剂为95%乙醇-99.5%丙酮(1:1,V/V)、浸提时间为8 h和浸提温度为21℃。
译  名:
Effect of Extraction Conditions on the Spectrophotometric Determination of Chlorophyll Content in Spirulina platensis Powder
作  者:
LUO Guang-hong;XU Yao-zhao;HE Lin;Gansu Engineering Research Center for Microalgae;Institute of Hexi Ecology and Oasis Agriculture,Hexi University;Science and Technology Department of Zhangye;
关键词:
Spirulina platensis;;spectrophotometry;;powder;;chlorophyll content
摘  要:
The aim of this study was to establish the optimal sample preparation conditions for the spectrophotometric determination of total chlorophyll,chlorophyll a and carotenoids in Spirulina platensis power with respect to three extraction parameters,extraction solvent,time and temperature. The results showed that the chlorophyll contents of Spirulina platensispowder determined with different extraction solvents decreased in the following order: 95% ethanol-99.5% acetone(1:1,V/V) > 95% ethanol-99.5% acetone(2:1,V/V) > 95% ethanol-99.5% acetone(1:2,V/V) > 99.5% acetone > 95% alcohol. The decreasing order was 8 h > 6 h > 4 h > 14 h > 12 h > 10 h > 24 h > 2 h for different extraction times,and 21 ℃ > 22 ℃ > 20 ℃ > 15 ℃> 25 ℃ for different extraction temperatures. The optimal extraction conditions of the determination of chlorophyll content in Spirulina platensis powder were found to be extraction 21 ℃ for 8 h using 95% ethanol-99.5% acetone(1:1,V/V) as the solvent.

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